e.2. What is the amount of each component needed, given the following mediacomposition per liter of distilled water:computationShow yourComponentg/Ltryptone10.0NaCI5.0yeast extract10.0agar15.0For tryptone:For yeast extract:For NaCl:For agar: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants(small test tube), and 15 stabs (big test tube) of Luria Bertani Agar.

Introduction Lysogeny Broth (LB) Is A Nutrient-dense Media Predominantly Used For Bacterial Growth.…

e.2. What is the amount of each component needed, given the following media composition per liter of distilled water: computation Show your Component g/L tryptone 10.0 NaCI 5.0 yeast extract 10.0 agar 15.0

e.2. What is the amount of each component needed, given the following media composition per liter of distilled water: computation Show your Component g/L tryptone 10.0 NaCI 5.0 yeast extract 10.0 agar 15.0

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter8: Basic Parasitology

Section8.2: Collecting And Processing Specimens For Parasite Detection

Problem 8RQ

See similar textbooks

Related questions

Q: 1. Prepare 30 mL of a 5 mg/mL oral phenobarbital solution using phenobarbital available from the…

A: Given values: Concentration of phenobarbital stock solution = 65 mg/ml Concentration of the…

Q: Describe, stepwise, how you would prepare 0.5 L of a 0.15 M phosphate buffer, pH 12.5, using…

A: The equilibrium between the weak acid and its conjugate base allows the solution to resist changes…

Q: Provide a chemical test/reagent that would differentiate each of the following pairs of compounds.…

A: Asked : Reagent and indication of positive test

Q: Calculate the cocentrations of acetic acid (pKa = 4.76) and sodium acetate necessary to prepare a…

Q: Balance the Alka-Seltzer tablet chemical equation and Identify the acid and base in the reaction.

A: Seltzer tablets neutralize excess stomach acid. These tablets dissolve in water when ingested. When…

Q: How much caffeine can be extracted using 50.0ml of DCM if a tea bag contains 15 grams of tea leaves…

A: Given Values: Amount of tea leaves = 15 grams % of caffeine present in tea leaves = 0.9% Amount of…

Q: You are told to prepare 200 mL of fixative solution containing 30% formaldehyde (vol/vol), 1X…

A: As we want 30% formaldehyde out of total 200ml so we take .3*200 = 60ml formaldehyde is taken.

Q: Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest…

A: Chromatography is a technique for separating different components of a sample, where the analyte is…

Q: You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar,…

A: Media is providing the nutrients for growth of micro organism.

Q: Complete the table below. Assume that the only reaction is dissolution. Ignore the effects of…

A: Asked : Solution for the given table

Q: Define sterols.

A: Lipids, as a class of compounds, are insoluble in water but are soluble in other organic solvents.…

Q: 50 mL of 5mM NaCl solution from 1 M NaCl solution

A: Dilution is the process by which we decrease the concentration of a solution. This process is vital…

Q: Beef extract Peptone Sodium chloride Agar Distilled water 3.0 g 5.0 g 8.0 g 15.0 g 1000 mL

A: Nutrient agar is the most common general-purpose medium, thats supports the growth of the wide range…

Q: What volume of 0.1M NaOH (reagent) needs to be added to increase your 7.5 pH (buffer) by 0.5 pH…

A: The buffer is a species which resist any change in pH on adding small amounts of an acid or base in…

Q: Now calculate the slope and equation for the best fit line. You may use excel

Q: DATA TABLE L6108.3 nm Lamda max Trial Concentration (mol/L) Absorbance 0.428 0. 8०० 1.17 1.498 1.845…

A: Spectroscopy is the interaction between electromagnetic radiation and matter. If transmittance T…

Q: Explain what method is used to identify the solid structure of the catalyst (catalyst…

A: The characterization of many catalysts involves a knowledge of the physical properties of the…

Q: Elemental gold is being recovered by electrodeposition from a solution with tiny amounts of Au(III).…

A: Gold is recovered by many process like cynide process etc.

Q: ect in ePortfolio idol™M → Caffeine Acetaminophen Solvent front Download 14. A student conducted an…

A: Introduction Amino acids are chemical molecules containing side chains unique to each amino acid as…

Q: What volumes of 50 mM CH3COOH and 30 mM NaCH3COO solutions are needed to prepare 2L of 20 mM acetate…

A: Given Values: The pH of acetate buffer = 3.6 Concentration and volume of acetate buffer = 20 mM and…

Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of…

A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle…

Q: Given log þ. 2. b. Balance the following reactions: S S(s) + NO(g) in acidic medium (aq) 8 (aq)…

A: Answeing 4b as you have asked. In acidic medium, protons will be there. The protons along with the…

Q: To prepare 500 mL of a 1X TAE buffer solution, we need to mix solution (so 25 times more…

A: TAE is a standard buffer for the preparation and operation of DNA agarose gels. TBE provides…

Q: Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B…

A: The medication employed for curing fever, inflammation is known as aspirin and it is a chemical…

Q: b) What is the function of using the following chemicals in the Determination of Zinc in…

A: In the Ion-exchange Separation, the dissolved ions are removed from the solution, and like charges…

Q: Prepare a 75 ml of a 10% (w/v) FeCl3 solution. How many grams of FeCl3 must be weighed out and…

A: Given Values: Volume of FeCl3 solution = 75 ml Concentration of the FeCl3 = 10% (w/v)

Q: Complete the following precipitation reactions using balanced chemical equations:

A: The reaction in which the formation of insoluble salt when two soluble salts mix together in the…

Q: You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris…

A: The mole is used as a unit in the SI system to quantify the amount of substance present. One mole is…

Q: Match the common laboratory scenarios to the correct buffer required using the guide below.…

A: Buffer It is defined as a solution that have the ability to resist change in pH upon the addition…

Q: (i) (iv) 3.0 2.5 2.0 1.5 1.0 0.5 0.3 (ii) (iii) Air 20°C 101525Pa Limin

A: Bioreactor: It consist of a vessel where biological reactions takes place through the transformation…

Q: Determine the fluoride ion concentration for a saturated solution of magnesium fluoride. K sp = 7.4…

Q: Consider a HCI solution with a pH of 4.70. a) Calculate the concentration of HCl. b) Identify the…

A: pH is the scale which is used to specify the acidity or basicity of an aqueous solution. Acidic…

Q: Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. for pH 6, α4 = 2,2 x 10-5

A: Ethylene diamine tetra-acetic acid (EDTA) is an aminopolycarboxylic acid with the formula…

Q: When counting WBCS, what is the best diluent? O Turk's solution O 0.85% NaCI O 70% alcohol O…

A: In the immune system, lymphocytes refer to the white blood cells that play an important role in the…

Q: Explain briefly effects and importance of following phenomena on bioreactor design. oxygen demand…

A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…

Q: In water content determination How many mL of water is expected from a 20 g Digitalis sample…

A: A drug, often known as a medicine, is a chemical compound that has a biological effect on the human…

Q: Prepare a complete analysis procedure using KMnO4 as teh oxidant instead of K2CrO7 in the redox…

A: Redox indicators have a defined color at a defined potential. Equivalence potential and indicator…

Q: How would I calculate the amount of each component in each solution? Stock solutions provided:…

A: In-order to perform dilution, we take a specific volume from stock solution and transfer it to…

Q: Complete the diagram below for the analysis of an unknown salt. Drag the appropriate reagent into…

A: Analysis of unknown salt by appropriate reagent is asked.

Q: f a 250 ml solution of ethanol in water is prepared with 4 ml of absolute ethanol (100%), what is…

A: Dilution factor It is defined as the ratio of the initial volume of a solute to the final volume…

Q: a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product…

A: Given values in the question are as follows: mAb = 120 mgl Total volume of the solution = 5 l…

Q: 5 L of 0.2 M dextrose solution from 5 M dextrose solution

A: Given Values: Stock solution of dextrose = 5 M Final solution of dextrose = 0.2 M Volume of the…

Q: Show calculations for making 10 mls each of the following dilutions of sucrose from the 0.30 M stock…

A: Dilution is defined as a term that refers to the solution that is made by adding solvent to a…

Q: Calculate the molarity of dilute Ca(OH)2 solution if the titration of 30.00 mL of 0.05231 M HCl…

A: Given : 30.00 mL of 0.05231 M HCl For this , n 2=1 as 1 hydrogen ion is present V2= 30 ml M2 =…

Q: Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions?…

A: For using persons square we need to make a square first then on the left side of the square we write…

Q: 950 mL of 1X CuSO₄ * 5H₂O solution from 25X CuSO₄ * 5H₂O solution

A: Given Values: Stock solution = 25 X CuSO4 . 5H2O Final Solution = 1 X CuSO4 . 5H2O Volume = 950 mL

Q: An aqueous antifreeze solution is 45.0% ethylene glycol () by mass. The density of the solution is…

A: Hello, thank you for your question. This question is a chemistry question but we are providing you…

Q: Determine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml…

A: Weight by volume or (w/v) solution contains the measured weight of the solids in the final volume,…

Q: Calculate the boiling points of a 8.50 m aqueous solution of fructose. Boiling point constants can…

A: Formula for boiling point: ∆Tb=Kbmwhere,∆Tb= change in boiling pointkb= boiling point constantm=…

Question

e.2. What is the amount of each component needed, given the following media
composition per liter of distilled water:
computation
Show your
Component
g/L
tryptone
10.0
NaCI
5.0
yeast extract
10.0
agar
15.0
For tryptone:
For yeast extract:
For NaCl:
For agar:

For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants
(small test tube), and 15 stabs (big test tube) of Luria Bertani Agar.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

What test can help you differentiate staphylococci from streptococci? What reagent or media would be use? short answer only
Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:
you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate method what other method could you choose to separate the bacteria? the result you will be looking for and interprete the result.
In a Kligler Iron Agar/Triple Sugar Iron test you end up with a result with a yellow butt and red slant. Please interpret this result. In the same test you end up with the agar mainly black but with a yellow slant what does this result indicate?
In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.
You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.
What is the difference between the pour plate method and the spread plate method? Why might you choose one over the other? What kind of plate must this be and why do you say that?
Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture