1. Peroxisomes are organelles surrounded by a single membrane. Soluble proteins that reside within peroxisomes are imported post-translationally, and they often contain the C-terminal tripeptide SKL (serine-lysine-leucine). a) How would you confirm that a C-terminal SKL tripeptide is a peroxisomal targeting signal? b) If cells were engineered to produce a hybrid protein consisting of a mitochondrial matrix protein (including its signal sequence) followed by a C-terminal SKL signal, where in the cell would you expect the hybrid protein to be found? Explain your reasoning.

1. Peroxisomes are organelles surrounded by a single membrane. Soluble proteins that reside within peroxisomes are imported post-translationally, and they often contain the C-terminal tripeptide SKL (serine-lysine-leucine). a) How would you confirm that a C-terminal SKL tripeptide is a peroxisomal targeting signal? b) If cells were engineered to produce a hybrid protein consisting of a mitochondrial matrix protein (including its signal sequence) followed by a C-terminal SKL signal, where in the cell would you expect the hybrid protein to be found? Explain your reasoning.

Name: 1. Peroxisomes are organelles surrounded by a single membrane. Solubl
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: 1. Peroxisomes are organelles surrounded by a single membrane. Soluble proteins thatreside within peroxisomes are imported post-translationally, and they often containthe C-terminal tripeptide SKL (serine-lysine-leucine).a) How would you confirm tha

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 10QP: If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense...

See similar textbooks

Similar questions

If an extra nucleotide is inserted in the first exon of the beta globin gene, what effect will it have on the amino acid sequence of the globin polypeptides? Will the globin most likely be fully functional, partly functional, or nonfunctional? Why?
Each intracellular fusion of a vesicle with a membrane requires a SNARE protein on the vesicle (called the v-SNARE) and a SNARE protein on the target membrane (called the t-SNARE). Assume that a genome encodes 21 members of the vSNARE family and 7 members of the t-SNARE family. With the assumption of no specificity, how many potential v-SNARE–t-SNARE interactions could take place?
A synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-Pro- Ile-Ser-Ala. What would be the effect on translation if the following components were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. a. Initiation factor 3 b. Initiation factor 2 c. Elongation factor Tu d. Elongation factor G e. Release factors RF-1, RF-2, and RF-3 f. ATP g. GTP
In Figure 1-8b, can you tell if the number of hydrogenbonds between adenine and thymine is the same as thatbetween cytosine and guanine? Do you think that aDNA molecule with a high content of A + T would bemore stable than one with high content of G + C?
A synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-ProIle-Ser-Ala. What would be the effect on translation if the following component were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. Q.Release factors RF-1, RF-2, and RF-3
1d) What amino acid would a tRNA with the anticodon 5'AGG3' carry?
Assume that during the process of creating the SHH protein an error was made. Instead of producing a 462-amino-acid-long protein, you only make a 20-amino-acid-long protein. Assume the error is detected in the Golgi apparatus. Will this protein be exported into the extracellular space? If your answer is no, what will happen to this protein?
The gene for a eukaryotic polypeptide 300 amino acid residues Iong is altered so that a signal sequence recognized by SRP occurs at the polypeptide's amino terminus and a nuclear localization signal (NLS) occurs internally, beginning at residue 150. Where is the protein likely to be found in the cell?
If an RNA molecule could form a hairpin with asymmetric internal loop, as shown in Figure Q6–5, couldthe complement of this RNA form a similar structure? If so,would there be any regions of the two structures that areidentical? Which ones?
Would increasing the concentration of salt prevent the mutant protein from aggregating?
Translate the following mRNA: 5-A U G A A A U U U C U U U A G G U C G A A -3 NH3+- Met-Leu-Phe-Val- COO- NH3+- Met-Thr-Val-Ser- COO- NH3+- Met-Glu-Gln-Ser- COO- NH3+- Met-Asp-Ser-Pro- COO- NH3+- Met-Lys-Phe-Leu- COO-
What are the four components for the translocation of protein across endoplasmic reticulum? and briefly explain ORF?