Demonstrate your understanding of industrial microbiology by explaining the involvement of in the following items, mentioning the type of microorganisms involved. microorganisms (i) AZD1222 vaccine (ii) (iii) Tempeh (fermented soybean) Kombucha
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- A student inoculates Escherichia coli into two test tubes containing the same sterile liquid medium, except the first tube also contains a drop of a chemical disinfectant with an antimicrobial effect. After 24 hours of incubation, the first tube remains clear while the second tube becomes cloudy with bacteria. How would you go about determining if the chemical is bacteriostatic or bactericidal?Identify the microorganism/ group of microorganisms involved in the spoilage of the following food: a. Salmon pink discoloration of sauerkraut b. Blue cottony growth on oranges c. Canned curdled evaporated milkOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginosWhat appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobCreate a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.
- what information about the identity and physiology of a bacterium or yeast can be learned from examing colonies of unknown microorganisms growing on solid media (agar)Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixtureMicroorganisms are often grouped according to their optimum growth temperatures. Which groups are most likely to spoil refrigerated foods?