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- 1. What are the different hoods in the lab and why are they important? 2. How does the light compound microscope magnify an object? Outline the process.1. Is a microscope an absolute necessity for studying microorganisms? Why or why not? 2. What do you think are the limitations of a brightfield microscope? Why is a brightfield microscope the most common type of microscope in routine clinical laboratories? 3. A slide in which a stained specimen is placed on the microscope stage. After careful and correct manipulation of the adjustment knobs, the specimen is still not in focus. What do you think is the reason? What corrective actions can be done? (provide computation) 1. What is the formula to determine the actual size of an organism? 2. The actual size of an Escherichia coli is 1.5um. What would be its image size if the magnification used is 1000x? 3. What magnification is used if an Bacillus sp. have a microscopic size of 3.5um and an image size of 15mm? NOTE: please try to answer all of the question, i promised to give you a good ratingg1. Name the part of the microscope that you use to control the cone of light coming through the condenser. 2. What is the total magnification of your specimen you are viewing using the 40X lens?
- 1. You're using a microscope with 10x magnification ocular lenses and a 25x magnification objective lens. What is the microscope's overall magnification at this setting? 2. Is it correct that immersion oil improves image magnification and resolution?A novice microbiology student incorrectly explains that immersion oil increases the magnification of his microscope. What is the function of immersion oil?1-What can occur if you quickly drop the coverslip onto your wet mount? 2-How can one add stain to the wet mount that has already been prepared? 3-Some live microorganisms can move very quickly. How do we slow these down to view in the microscope?
- 1. The resolving power of a microscope is a function ofa. the magnifying power of the lenses.b. the numerical aperture of the lenses.c. the wavelength of light.d. Both (a) and (b) are correct.e. Both (b) and (c) are correct.1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?1. What three things change as you increase magnification? 2. How do you properly use a microscope?
- 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?3. What are the ways in cleaning the objectives of the Microscope after use? (Write your answer below)3. Explain the following terms and what are its corresponding requirements for Raman applications: a. Confocal microscope b. Numerical aperture c. Infinity correction d. F-number