1. Which of the following bacterial species is not naturally competent? A) Bacillus subtilis B) Haemophilus influenzae C) Escherichia coli D) Streptococcus pneumoniae

Human Anatomy & Physiology (11th Edition)
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1. Which of the following bacterial species is not naturally competent?
A) Bacillus subtilis
В) Наеторhilus influenzae
C) Escherichia coli
D) Streptococcus рпеuтoniae
2. Bacterial species which are not naturally competent can be made competent in the laboratory by using
a combination of metal ions and temperature changes.
A) true
B) false
3. If 10 ng of plasmid DNA yielded 10,000 transformants per milliliter (mL), the transformation
efficiency is:
A) 105
В) 105
C) 107
D) 105
4. Under optimal conditions, all the bacterial cells in a sample can be transformed.
A) true
B) false
5. The plasmid called pGal, carries a gene encoding the enzyme beta-galactosidase. What is the natural
function of this enzyme?
A) It protects cells from a specific type of antibiotic.
B) It hydrolyzes the disaccharide lactose.
C) It is involved in plasmid replication.
D) It is necessary for transformation.
6. The pGal plasmid also carries a gene designated as amp". What is the function of this gene?
A) It protects cells from a specific type of antibiotic.
B) It hydrolyzes the disaccharide lactose.
C) It is involved in plasmid replication.
D) It is necessary for transformation.
7. Which of the following is a substrate for the beta-galactosidase enzyme?
A) lactose
B) sucrose
С) Х-Gal
D) IPTG
E) both A and C
Transcribed Image Text:1. Which of the following bacterial species is not naturally competent? A) Bacillus subtilis В) Наеторhilus influenzae C) Escherichia coli D) Streptococcus рпеuтoniae 2. Bacterial species which are not naturally competent can be made competent in the laboratory by using a combination of metal ions and temperature changes. A) true B) false 3. If 10 ng of plasmid DNA yielded 10,000 transformants per milliliter (mL), the transformation efficiency is: A) 105 В) 105 C) 107 D) 105 4. Under optimal conditions, all the bacterial cells in a sample can be transformed. A) true B) false 5. The plasmid called pGal, carries a gene encoding the enzyme beta-galactosidase. What is the natural function of this enzyme? A) It protects cells from a specific type of antibiotic. B) It hydrolyzes the disaccharide lactose. C) It is involved in plasmid replication. D) It is necessary for transformation. 6. The pGal plasmid also carries a gene designated as amp". What is the function of this gene? A) It protects cells from a specific type of antibiotic. B) It hydrolyzes the disaccharide lactose. C) It is involved in plasmid replication. D) It is necessary for transformation. 7. Which of the following is a substrate for the beta-galactosidase enzyme? A) lactose B) sucrose С) Х-Gal D) IPTG E) both A and C
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