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1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20 moles.channel-2.sec-1 Show me how they got their answer and explain how?

1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20 moles.channel-2.sec-1 Show me how they got their answer and explain how?

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 14QP
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1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20 moles.channel-2.sec-1 Show me how they got their answer and explain how?
e AutoSave OFF Insert Draw Design Layout X • Α' Α' Calibri (Bo... B I U ab X₂ X² CONTROL CFTR- 14 CRISPR f1 202 words IX V (mv) You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting the AF508-CFTR mutation. The vector uses CRISPR-CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the AF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR). The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10μA) conditions: AVRE (mv) Baseline Amiloride -78 -2 8 16 800 1600 5.7777 98 1 -38 -6 9 27 900 2700 42 2 R(S2.cm-2) I(μA.cm²) Vte (mv) AVRE (MV) References » Tell me Aa | A AA. R(S.cm-2) Document13 A Paragraph Styles tv Focus E 2 Forskolin -4 -23 Comments 6 600 D 38 Dictate Sensitivity = Inh172 (uA.cm2) Immunostaining using Quantum Dot fluorescence reveals that there are 5 CFTR channels/μm² in the "CONTROL" and 81 CFTR channels/μm² in the "CFTR-CRISPR". Calculate the number of moles of Cl- transported per channel per second for both treatments. Note that there are 1x108 μm² in each cm² -3 13 1300 2 -9 11 1100 Editing 15 8 C E Edit
Transcribed Image Text:e AutoSave OFF Insert Draw Design Layout X • Α' Α' Calibri (Bo... B I U ab X₂ X² CONTROL CFTR- 14 CRISPR f1 202 words IX V (mv) You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting the AF508-CFTR mutation. The vector uses CRISPR-CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the AF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR). The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10μA) conditions: AVRE (mv) Baseline Amiloride -78 -2 8 16 800 1600 5.7777 98 1 -38 -6 9 27 900 2700 42 2 R(S2.cm-2) I(μA.cm²) Vte (mv) AVRE (MV) References » Tell me Aa | A AA. R(S.cm-2) Document13 A Paragraph Styles tv Focus E 2 Forskolin -4 -23 Comments 6 600 D 38 Dictate Sensitivity = Inh172 (uA.cm2) Immunostaining using Quantum Dot fluorescence reveals that there are 5 CFTR channels/μm² in the "CONTROL" and 81 CFTR channels/μm² in the "CFTR-CRISPR". Calculate the number of moles of Cl- transported per channel per second for both treatments. Note that there are 1x108 μm² in each cm² -3 13 1300 2 -9 11 1100 Editing 15 8 C E Edit
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