The primary stain used in the endospore stain is:    A. Carbolfuchsin Stain B. Methylene  Blue C. Malachite green D. Safranine

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: The process that most accounts for magnification is a. a condenser c. illumination b. refraction of…

A: Magnification means the image produced is more than the size of the object.

Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A:

Q: c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain.…

A: Stain is a compound or dye which is used to impart colour for the bacterial cell surface so that we…

Q: A microscope that has a total magnification of 1,500× when using the oil immersion objective has an…

A: The microscope is the major tool in the identification of a microorganism after its isolation. The…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…

A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…

Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…

A: Staphylococcus aureus It is a Gram positive coccus bacteria. These bacteria have the very distinct…

Q: 3. Draw the specimen observed under endospore staining  4. Draw the specimen observed under…

A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…

Q: what is bibulous paper used for? a. bleaching the benchtop b. cleaning microscope lenses. c.…

A: To view sample sections under microscopes, slides should be prepared. For the preparation of slides,…

Q: For post mortem stain to occur ______must happen. A. Coagulation B. Hemolysis C. refridgeration…

A: For post mortem stain to occur pH shift to alkaline   must happen.

Q: The sediment observed in stained blood smear caused by: a) Overstraining b) More Haematoxylin in the…

A: Here we have to explain about why sediment observed in stained blood smear.

Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…

A: Introduction  Capsule is the outer most covering, which is present even outside the cell wall. This…

Q: Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe…

A: 1. Glass slides on which the sample is placed. 2. Cover slip made up of plastic. 3. Dropper 4.…

Q: What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b:…

A: According to bartleby expert guidelines, when multiple sub part questions are posted we are allowed…

Q: It is desirable that the microscope objectives be parfocal because the specimen will be…

A: Microscopic organisms are small organisms that are not visible to the naked eye and can be seen only…

Q: Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining…

A: The correct option is . A Gram's Staining procedure

Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…

A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…

Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…

A: To answer this question first, we need to understand what is gram staining and what are the steps of…

Q: b. Successfully completed most steps of the Gram stain but failed to add the iodine (the mordant):…

A: Gram staining is a differential staining procedure used to classify two types of bacteria on the…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…

A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…

Q: Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary…

A: Differential staining uses more than one type of stains for staining the microorganisms and it is…

Q: Types of Differential Staining techniques.  1. Gram stain 2. Acid-fast stain     Please…

A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…

Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: Stains Primary Stain Mordant Destain Secondary Stain Diagram of Stain Simple Stain N/A N/A N/A Gram…

A: This question is regarding different staining techniques of microorganisms.

Q: Give three situations where the negative staining procedure would be used.

A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…

Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

A: The danish Bacteriologist Christian gram discovered this differential technique in order to…

Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium

A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…

Q: You make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to…

A: The staining procedure is a multi-step process that involves the smear preparation followed by…

Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: In order to have a better visualization of the shape acid the fast stain, you should do the…

A: Use of carbol fuchsin on the bacteria and heating it p, makes the cell appears red, presence of…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: This part of the staining process helps the primary stain to remain in the cell during…

A: Staining of biological samples is a very useful process for detailed observation and measurement of…

Q: Which of the following is not an aim of fixation? A. To prevent putrefaction B. Remove excess…

A: Tissue fixation is one of the steps of sample preparation for the observation under an advanced…

Q: Blood is stained with stain. a) Methylene blue b) Safranin c) Leishman stain d) Carbol fuchsine

A: Introduction - A blood smear, also known as a peripheral blood smear or blood film, is a thin…

Q: if there are features of the gram stain that are not as expected what possible sources or error may…

A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…

Q: Why might gram positive bacteria incorrectly appear to be gram negative after the gram staining…

A: Gram staining is a procedure which is used to identify whether the bacteria being tested in gram…

Q: Gram straining procedure. Indicate the following information A. Shape........ B. Stained color…

A: On the basis of gram staining, we can differentiate between two types of bacteria, one with thick…

Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…

A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.

Q: What are the expected result after gram staining?   a. Crystal Violet = b. Gram Iodine =  c.…

A: Gram staining is a type of staining technique used to differentiate bacteria based on their cell…

Q: why is gram staining important

A: Gram staining is a laboratory technique which is used to differentiate between different types of…

Q: Formation and Composition of Film from Milk a. Place 10 mL of fresh milk in an evaporating dish.…

A: Milk  It is a nutritious white liquid secreted by the mammary glands of mammals. It consists of…

Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…

A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…

Q: Explain how bacterial cells would look in the gram staining procedure if the following mistakes were…

A: Microorganisms are those small organisms that are invisible to the naked eye and can be seen only…

Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…

A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…

Q: What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic…

A: Simple staining: * The process staining the bacterial cell with a dye  to see bacterial structure…

Q: The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain…

A: Bacillus anthracis is a Gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.