Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: Please discuss why staining is important in visualizing microbes under the microscope. What…
A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…
A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?
A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?
A: Cell staining is a technique that is used to better visualize cells and cell components under a…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…
A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: Define the term gram staining.
A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…
Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: What is the theory about the mechanism of the Gram-stain reaction? please include…
A: The mechanism of gram staining is employed essentially to differentiate between gram-positive and…
Q: ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use…
A: The Gram stain is a differential staining method used for classifying bacteria into either…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?
A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…
A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: Explain briefly why the 100X objective lens of the light microscope is immersed in oil.
A: The ability of a microscope to reveal an image that is numerous times larger in size than the…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: do we need to stain microorganisms?
A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
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Solved in 3 steps
- Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)When it comes to simple stains, why is time so important? And what is the purpose of rinsing the slide in water after each Gram staining procedure?Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?
- In preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?How are negative stains prepared?Name two applications for which this type of stain especially useful?
- Please discuss why staining is important in visualizing microbes under the microscope. What different types of staining methods can be used?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?what are the positive and negative results of the endospore stain procedure?
