10. a) What is Lambert-Beer Law? What is the relationship between absorbance and concentration? Does absorbance have a unit? Explain. b) Explain the terms below: • Chromophore: • Auxochrome: • Bathochromic shift: • Hypsochromic shift:
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- Does a calibration curve alwasy compare concentration and absorbance? Or can it compare concentration and %T as well?1. how aas work? 2. how uv-vis work? 3. how nmr work? 4. how infrared spectroscopy work? 5. how molecular spectroscopy work?Project 2: Food Dye Spectroscopy How can you use serial dilutions to determine the relationship between concentration and absorbance? Graphs may also help in this explanation.
- Q1. Why is it important to acquire a reference spectrum and subtract it from the sample’s spectrum? Q2. Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)? Q3. What would happen if you put too much of a compound in the cuvette during the spectral measurement? Q4. Why is it important to avoid any scratch on the cuvette? Q5. Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not? Q6. Predict the maximum absorption wavelength for 2,4-heptadien-6-one using Woodward-Fieser rules. Q7. Why are n-π* transitions generally of low intensities/low absorptions? Q8. What photon energy and wavelength would you need to send on a compound having an energy gap (Eg) of 2.7 eV? Detail your answer with appropriate information. Q9. Using molecular orbital theory/concept (draw the important molecular orbitals), explain the difference in absorption for the…This is about Beer-Lambert’s Law and UV-vis Spectroscopy: 1. How are concentration and absorbance related?2. How can you determine the concentration of an unknown sample?How do I do question #3 about correspondence between % transmittance and absorbance? Thank you
- The beer-lambert law is A = εlc, where A is the absorbance, ε is the molar extinction coefficient, l is the path lengh in cm and C is the concentration. The beer lambert law can be expressed in the form of y=mx + b, where A is y, and C is x.What is b and how is it used to calculate the final absorbance?What would be the multiplicity associated with peak 2?Why is it necessary to run a blank before measuring the absorbance of a sample? To allow the lamp and electronics of the spectrophotometer to warm up before taking the sample absorbance reading. So that scattered light will be filtered before hitting the photodetector. To account for the absorbance caused by the cuvette and the solvent To account for the absorbance caused by the cuvette alone .
- 10) Which type of spectroscopy would be best/easiest to distinguish between these two compoundsDo your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not? b) why does absorbance data relate more conveniently to concentration that does transmission data?Pls help to analyze this IR spectrum