10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.a. ActivationlactoseCAPlac lrepressorallolactoseCAMPCRNAPolpromoter operator+1lac Zlac Ylac ADNA lactoseb. RepressionCAPlac IrepressorallolactoseCAMPCRNAPolpromoter operator+1lac Zlac Ylac ADNA

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Description: 10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.a. ActivationlactoseCAPlac lrepressorallolactoseCAMPCRNAPolpromoter operator+1lac Zlac Ylac AD

The prokaryotic gene regulatory system is known as operon system in which the expression of…

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Biology

10. Modify the diagrams below to reflect the activation and repression of lac operon. Use arrows, lines, X's, etc. to illustrate control of gene expression. lactose a. Activation CAP lac I repressor allolactose CAMP с RNA Pol promoter operator +1 lac Z lac Y lac A DNA

10. Modify the diagrams below to reflect the activation and repression of lac operon. Use arrows, lines, X's, etc. to illustrate control of gene expression. lactose a. Activation CAP lac I repressor allolactose CAMP с RNA Pol promoter operator +1 lac Z lac Y lac A DNA

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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The a/a operon is an inducible operon that controls the production of the sugar arabinose. When arabinose is present in a bacterium it binds to the protein AraC, and the complex binds to the initiator site to promote transcription. In this scenario, AraC is a(n). activator inducer repressor operator
A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.
1. What is the default state of the lac operon? a. LacI repressor is sitting on the operator sequence and blocks RNAP from transcribing lacZYA. b. LacI repressor is not in a correct shape to bind to the operator sequence. c. LacI repressor is not produced in the absence of lactose. d. Allolactose is sitting on the operator sequence and blocks RNAP from transcribing lacZYA.
1) Assume you have an operon that is repressible. In this case, the rate of synthesis of repressor protein must depend on the level of a) co-repressor b) inducer c) both d) neither
1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production
1. A)Explain the mechanism by which the lac operon is sensitive to glucose levels (presence and absence of glucose). B)If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
4. What are the effects of the following conditions on Lac operon of bacteria?A)Glucose is absent and lactose is presentB)Glucose is present and lactose is presentC) Glucose is present and lactose is absent
1. If the above gene is one of the three structural genes of the lac operon that codes for theprotein/ enzyme responsible for breaking lactose into two molecules of simple sugars,a.) what triggers the activation of this gene?b.) what triggers the inactivation of this gene?c.) what substance is attached to the operator region of the operon in the absence ofactivator?d.) what gene is responsible for the synthesis of the substance used to attach in theoperator region in the absence of activator? e.) what substance is attached to the promoter region of the operon in this case?
4) Suppose you have an E. coli in which the gene that codes for the lac repressor protein has been mutated in such a way that the protein produced can bind to both lactose and DNA (at its specific binding site in the lac operon promoter) simultaneously. What effect, if any, would this have on regulation of expression of the lac operon? Briefly (1 sentence) explain why you would predict the effect or lack of effect this mutation would have.
1. In DNA amplification, using the Taq polymerase, what is the maximum number of amplification cycles? a. 30 b. 20 c. 60 d. 10 2. In the case of trp operon, for transcription to fully occur, which of the following conditions must be met? a. Environmental tryptophan level must be low. b. Environmental tryptophan must be absent. c. Environmental tryptophan level must be abundant. d. Both a and b are correct.
2) Name and describe two types of constitutive mutants of the lactose operon.