10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul trypan blue dye. The dilution factor of the square is 10^4. Determine the cells/mL using hemocytometer
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10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul trypan blue dye. The dilution factor of the square is 10^4. Determine the cells/mL using hemocytometer.
Rule:
Only count the cells on the top and left lines of square.
Based on the given figure, what is the cell concentration?
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- If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?Complete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9Based on these blood agar results, can you identify what type of hemolysis are occurring in these tests? Gamma, beta, or alpha? Not gamma, not beta, not alpha?
- Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in tube-A, then transferred 10 µL to the hemocytometer and started counting cells, then realized the cells were too crowded to count accurately, so Steven took an aliquot of 20 µL cells of cells from tube-A and mixed with 180 µL of cell culture media, transferred 10 µL onto the hemocytometer, and countered 500 cells from the squares on the four corners of the hemacytometer. What is the # of cells per mL? Please show all calculation steps.A bacterial suspension was diluted and 0.1 mL of each of the dilutions was plated in duplicate. The following results were obtained. What was the original concentration? Dilution CFU Counted CFU Counted 10-2 29 24 10-3 1 3 10-4 0 0A cell count was performed on a serous fluid specimen. As the fluid was cloudy, a 1:5 dilution was prepared. The counts were: 34 and 32; 5 squares on each side were counted. What is the average cell count per L? Side 1: 34 x 1 x (1/(5x0.1)) x 5 = 340 per µl Side 2: 32 x 1 x (1/(5x0.1)) x 5= 320 per µl
- The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mLA sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.