A urine sample with more than 100,000 organisms is considered indicative of infection. A urine sample containing 500 bacteria with a generation time of 20 minutes sits for 6 hours before finally being assayed. How many bacteria will then be present within the sample?
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- The culture primarily contains nutrients such as sugars and a gel (agar). Why don’t viruses grow in a culture like bacteria do? Explain.The below photograph shows a Gram-stained slide viewed using a light microscope set at brightfield of a clinical sample from a patient with symptoms suggesting an infection. The slide was viewed with x100 objective. What could be the most likely cause of infection? Gram positive bacteria, either rods or cocci Gram positive rod shaped bacteria or yeast Gram negative rod or cocci shaped bacteria Gram negative bacteria, either rods or cocciWhich of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination test
- True or False: Confirmatory identification is the final identification of a bacterium.Which of the following methods can identify different strains of a microbe? a. microscopic examination b. radioimmunoassay c. serotyping d. agglutination testWhat method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreader
- Why is the Widal Test performed? What disease does it indicate? Is the Widal test reliable? Enumerate the different bacteria that can cause false positive and false negative Illustrate how the antigens aggregate in the Widal TestWhich of the following would suggest your stock culture is contaminated? Which of the following would suggest your stock culture is contaminated? Gram stain indicates Gram-positive bacilli. Broth media become turbid. Gram stain indicates both Gram-positive cocci and Gram-negative bacilli. Colony morphology is uniform on the streak isolation plate. Gram stain indicates Gram-positive staphylococci.Samples from an ill patient were collected by a physician for testing. A bacterial infection is suspected. Using the unknown flowcharts, a microbiologist conducted various tests to determine the identity of the unknown bacterial isolate. The resulting data is included in this presentation. Identify each test.
- As a medical microbiologist, if you are presented with a patient suspected to have a bacterial disease, describe the various steps you would follow, to establish a laboratory diagnosis. Please keep brief - 8 sentences/dot points max.A microbiologist sets up a serial dilution to analyze bacteria in raw milk. Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3?A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?