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175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer?

175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer?

Introduction to General, Organic and Biochemistry
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter30: Nutrition
Section: Chapter Questions
Problem 30.3P: If sodium benzoate, a food preservative, is excreted as such and if calcium propionate, another food...
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Please solve for E and please show all calculations
● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCl pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide I 2% (v/v) Bis 2M Tris-HCl pH 8.9 Determine how much you need of each stock solution and dH₂O to prepare desired 50ml mix. e) 175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer? f) 6L of 1mM IPTG. You need to prepare a stock solution of 1M IPTG that will be sufficient to give you 6L of 1mM IPTG once it is diluted. How much water and how much IPTG (in grams) will you need to make a sufficient 1M stock solution?
Transcribed Image Text:● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCl pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide I 2% (v/v) Bis 2M Tris-HCl pH 8.9 Determine how much you need of each stock solution and dH₂O to prepare desired 50ml mix. e) 175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer? f) 6L of 1mM IPTG. You need to prepare a stock solution of 1M IPTG that will be sufficient to give you 6L of 1mM IPTG once it is diluted. How much water and how much IPTG (in grams) will you need to make a sufficient 1M stock solution?
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