1)Explain the peak order based on the structure of the compounds in an hold: paracetamol, acetylsalicylic acid and caffeine. 2) What influence would a change in the PH value have on the retention time and peak shape of these compounds?
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- Briefly describe how a phase-contrast microscope work and the kind of image that itproduces. Give a specific use for this type of microscope.Label base peak and molecular ion peak C4H10OA 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. To get the concentration of amine in this compound, you subject it to spectroscopic analysis. So, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample then you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. Solve for the molecular weight of the compound. The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.
- QUALITATIVE DETECTION OF ORGANIC COMPOUNDS Would you say that all organic compounds give evidence of containing carbon when they are burned or heated?A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.Why is it important for solutions to be diluted before they are run through spectroscopy?
- A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.2) In a laboratory study, a calibration was performed using NH4C1 to analyze ammonium spectrophotometrically. The results of the study are given below. According to this, when the 5.0 mL sample containing ammonium is completed to 50 mL with pure water, find the amount of NH4 in the sample whose absorbance was found as 0.12 according to the table. (N: 14 g / mol, H: 1 g / mol, Cl: 35.5 g / mol). Concentration (mg/L) Absorbance 0.01 5 0.05 7.5 0.1 10 0.15 12 0.18As the pH changes, how will the absorbance change at the wavelength of the isosbestic point?