In a laboratory study, a calibration was performed using NH4C1 to analyze ammonium spectrophotometrically. The results of the study are given below. According to this, when the 5.0 mL sample containing ammonium is completed to 50 mL with pure water, find the amount of NH4 in the sample whose absorbance was found as 0.12 according to the table. (N: 14 g / mol, H: 1 g / mol, Cl: 35.5 g / mol). Concentration (mg/L) Absorbance 0.01 5 0.05 7.5 0.1 10 0.15 12 0.18
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- What does a refractometer reading of 2 mean when distilling ethanol?A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. You subject it to spectroscopic analysis, in order to get the concentration of amine in this compound. Next, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample, and you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. What is the molecular weight of the compound? (The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.)A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound?
- A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound? I need help with part B of this question.Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus concentration (in M) of known solutions and finds the following slope and intercept: y = 1.646x + 0.026 Sally finds that her original Kool-aid sample is too concentrated, so they dilute it by transferring 13.00 mL of the original Kool-aid sample into a new container and diluting to a total volume of 37.00 mL. If the absorbance of the dilute Kool-aid sample at the appropriate wavelength for their assigned dye is 0.950, determine the concentration (in M) of the assigned food dye in the original Kool-aid sample. Report answer with three places after the decimal.A compound whose molecular mass is 158 g / mol has a molar absorptivity of 3.57 x 104 . How many grams of compound must be dissolved in 1 L of solution so that after diluting it 200 times, the solution has a absorbance of 0.350, in 1 cm cell?
- Chemistry The most abundant type of mammalian antibody circulating in the blood stream is IgG. A solution prepared from 0.798 g of a particular IgG in 250 mL of water shows an absorbance of 0.578 at 275 nm in a 1.00 cm cell. If the molar absorptivity of the IgG is 6.49 × 104 M–1 cm–1 at 275 nm, what is the molar mass of the IgG? Present your numerical answer in kg·mol-1 to 3 significant figures .Caffeine (C8H10O2N4 • H2O) has an absorbance of 0.510 at 272 nm and 1 cm optical path in1 mg / 100 mL concentration solutions. A 2.5 g sample of soluble coffee is diluted withwater to 500 mL. Take 250 mL, add 25.0 mL of 0.1 N H2SO4 and dilute it to 500 mL. Theabsorbance at 272 nm resulting in 0.415. A) Calculate the grams of caffeine per kg of soluble coffee in the sample. Themolar mass of caffeine is 212 g / mol.The nitrite in a series of standard solutions (mg/L, n = 5) are converted to azo dye and the slope of the calibration curve is 2.0 ppm1. A 10.00-mL mineral water sample is treated in the same way as standards and diluted to a final volume of 100.00-mL. It gives an absorbance of 0.80. The absorbance of blank solution under the same conditions is 0.10. Calculate ppm (mg/L) of NO2 (46 g/mol) and the molarity of NANO2 (69 g/mol) in the original sample
- A plot of Absorbance vs. molarity of Fe(SCN)2+ from part one of the lab gives a straight line with the following equation. y = 51x R2 = 0.998 If a student measures an absorbance value of 0.375 in the second part of the lab, what is the molarity of Fe(SCN)2+ in this solution?After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40 and processing her data, Sally found that her drinking strength Kool-Aid sample had an FD&C Red 40 concentration of 1.086 x 10–5 M. If 0.133 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her Kool-aid powder?The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the instrument returned an error because the absorbance was too high. The sample was then diluted by using a pipette to take 100.0 μLμL of the sample and injecting it into a cuvette already containing 2.00 mLmL of water (total volume is 2.00 mLmL + 100.0 μLμL). The absorbance value of the diluted solution corresponded to a concentration of 7.71×10−6 M M . What was the concentration of the original solution? Express the concentration to three significant figures with the appropriate units.