2. As we've seen previously, peptide bonds involving the amino acid proline can populate both "trans" and "cis" isomers. trans-Pro cis-Pro This isomerization reaction can be the rate-limiting step in protein folding. Depending on the protein sequence, the relative favorability of the trans and cis states can change. For Pro-117 in the protein staphylococcal nuclease, the rates of the forward and reverse reactions are: ktrans-cis = 3.1 x 10-2 and keis→trans = 2.5 x 10-3 s-1. (a) What is AG" for this reaction? (b) A prolyl isomeriase enzyme increases the rate of the forward reaction to kcat,trans+cis = 2.6 s-. What is the new rate constant for the reverse reaction (kcat,cis→trans)? = 2.6 s-1, KM = 1.9 µM. If (c) This prolyl isomerase is a Michaelis-Menten enzyme with kcat you have 1 nM enzyme, what is Vo for [S]o = 0.7 µM? What about [S]o = 30 µM? For each condition, what limits the reaction speed?
2. As we've seen previously, peptide bonds involving the amino acid proline can populate both "trans" and "cis" isomers. trans-Pro cis-Pro This isomerization reaction can be the rate-limiting step in protein folding. Depending on the protein sequence, the relative favorability of the trans and cis states can change. For Pro-117 in the protein staphylococcal nuclease, the rates of the forward and reverse reactions are: ktrans-cis = 3.1 x 10-2 and keis→trans = 2.5 x 10-3 s-1. (a) What is AG" for this reaction? (b) A prolyl isomeriase enzyme increases the rate of the forward reaction to kcat,trans+cis = 2.6 s-. What is the new rate constant for the reverse reaction (kcat,cis→trans)? = 2.6 s-1, KM = 1.9 µM. If (c) This prolyl isomerase is a Michaelis-Menten enzyme with kcat you have 1 nM enzyme, what is Vo for [S]o = 0.7 µM? What about [S]o = 30 µM? For each condition, what limits the reaction speed?
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter15: From Dna To Protein
Section: Chapter Questions
Problem 4TYK
Related questions
Question
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 2 images
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax