2. Below are images showing the cell envelopes of Gram-positive and Gram-negative bacteria. Indicatewhich one is Gram positive and which one is Gram negative. Following Gram staining, what would be thecolor associated with each type of cell? Also write the names of the structures to which the lines arepointing.(A) Gramcolor of cells after Gram stain:(1)(2).(3)50 nmMoobiskyy AnEverimy Kience Third EHilien Figure 3.15.02SCOURTESY OF BENOIT ZUBER/MRC.O ORYOF MOLECULAR BIOLO (B) Gramcolor of cells after Gram stain:(1)(2)50 nmMerndiolagy An Ereving Science Thrd Edtion higure 31501(C) Name the microscopy technique used to capture these images.gcOURTESY OF BENOIT ZUDER/MRCLABORATORY OF MOLECULAR BIOLOGY

Introduction - Microbiologist Christian Gram created a test in 1884 to determine whether a bacterium…

2. Below are images showing the cell envelopes of Gram-positive and Gram-negative bacteria. Indicate which one is Gram positive and which one is Gram negative. Following Gram staining, what would be the color associated with each type of cell? Also write the names of the structures to which the lines are pointing.

2. Below are images showing the cell envelopes of Gram-positive and Gram-negative bacteria. Indicate which one is Gram positive and which one is Gram negative. Following Gram staining, what would be the color associated with each type of cell? Also write the names of the structures to which the lines are pointing.

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter13: Diversity Of Microbes, Fungi, And Protists

Section: Chapter Questions

Problem 1ACQ: Figure 13.6 Which of the following statements is true? a. Gram-positive bacteria have a single cell...

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4. What is the theory about the mechanism of the Gram-stain reaction? 5. What part of the bacterial cell is most involved with Gram staining, and why? Please provide a reference
1. What name is given to the process of endospore formation in a bacterial cell? 2. How long is a smear heated in the heat-fix step prior to an endospore stain?.
1. You accidentally used safranin as the primary stain and malachite green as the counter stain during a spore staining procedure. Explain how your microscopic observation would differ from those observed when slides of spore-containing bacteria were prepared correctly. 2.One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found? What errors could have contributed to the variable result?
1. why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stain 2.What is the purpose of the bacterial capsule?
a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?
6)Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.
The Gram staining (Ex. 3-6) procedure is exactly the same for Gram-positive and Gram-negative bacteria cells. So, why do the Gram-positive and Gram-negative cells end up looking very different after performing the Gram stain? (In your answer, explain the differences between the Gram-negative and Gram-positive bacteria cell wall structure and explain how the alcohol reacts differently with both the Gram-positive and Gram-negative bacteria cell walls.)
2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?
5) Why are the streak plates made from a sample of coconut water incubated at 7°C?
1.why is gram stain considered a differential stain? 2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?
1. A student is directed to make a simple stain of coli with crystal violet, but got mixed up and used safranin instead. How would their observations be different? Would the information obtained be any different? 2. How is the procedure different when taking cells from a solid medium compared to taking cells from a liquid medium? Why is it important that your smear be thin?
1) In Gram staining, to what cell structure do the dyes bind? 2) Would it be useful to perform a Gram stain on a mixed culture? Why? 3) In capsule staining, why does the capsules did NOT take in any dye? 4) In endospore staining, what is the purpose of using the steam?