2. Draw an already streaked agar plate on the circle provided below with bacterial growth. If your goal is to harvest bacterial cells for DNA extraction, where would you likely get your bacterial sample? Encircle the area where you will do your extraction.
Q: A mixture of sand and organic matter is poured with a solution containing a positive dye (violet),…
A: C. The leachate has the same color (violet) with that of the dye used cause the organic matter…
Q: 2. How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Note: Ad per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction: The…
Q: student is directed to make a simple stain of coli with crystal violet, but got mixed up and used…
A: Bacteria cell walls are stained by the crystal violet. Iodine is subsequently added as a mordant to…
Q: Why must m-Endo broth or some similarly selective and differential medium be used to count…
A: Coliform bacteria are described as rod-shaped, Gram-negative, nonspore-forming, motile or nonmotile…
Q: 1. A term of biologists performed an experiment to determine the effectiveness of a chemical to…
A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…
Q: n't we say "sterile" technique?
A: To get the remaining sub-parts solved, please repost the complete question and mention the sub-parts…
Q: 2. Below are images showing the cell envelopes of Gram-positive and Gram-negative bacteria. Indicate…
A: Introduction - Microbiologist Christian Gram created a test in 1884 to determine whether a bacterium…
Q: If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL),…
A: Introduction Cytometry is referred to the study of the measurement of the characteristic of the cell…
Q: 2. The following is an incomplete schematic diagram for the isolation procedure used. Label each…
A: Isolation of Micro organisms -- Introduction --The term isolation is defined as separation of a…
Q: 13. Which of the following is NOT the part of growth medium for animal culture?
A: Animal cell culture is the process by which cells are grown in an artificial environment, outside of…
Q: 1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2)…
A: The staining techniques are used to identify the morphological characteristics along with the size…
Q: 3) The use of the Gram stain in microbiology is important because it differentiates A) Bacteria from…
A: Microbiology is the study involved in the microscopic organisms like bacteria,viruses,archaea ,fungi…
Q: 3. Why is it important to start a bacterial culture with a single, isolated colony?
A: A bacterial culture is the one which contains nutrient media required for the growth of bacteria.
Q: d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you…
A: 2d) In the initial diagram given, the number of colonies from tube 4, that were formed on the plate…
Q: Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How…
A: Introduction :- CFU( colony forming units ) are the count of cells present in orginal culture which…
Q: What is the statistically significant number range of colonies that may be counted on a Petri dish…
A: 1. 30-300 Sorry but as per Bartleby policy i can only solve first part. Please can you upload the…
Q: 2. Determine the number of bacterial cells per ml. in the original culture. Iml 1ml 1ml 1ml 99ml…
A: The CFU stands for colony forming units. The number of bacteria per ml is calculated by: (Colony ×…
Q: 3. You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…
A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…
Q: Which of the following best explains why there is no growth on plate II? (a) The initial bacterial…
A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…
Q: 1. What are the advantages of staining a bacterial preparation before observing it under a…
A: Since you have asked multiple questions, we will solve the first three questions for you. If you…
Q: 9. You make a 2 ml starter culture of a strain of E coli you typically work with. You take 100 µl of…
A: Given Values: (9) 10 μl of the culture is taken which is dissolved in 990 μl PBS buffer. So, the…
Q: 1. What other test can be used to isolate, differentiate and enumerate Bacillus cereus from food…
A: The Bacillus cereus group is found in abundance in both nature and food. Several members of this…
Q: You dilute the suspension to 10-4 and plate 100ul onto an agar plate. After overnight incubation…
A: Culture refers to the technique by which micro-organisms are grown in the specific medium rich in…
Q: 6. In the beginning the cell suspension is isolated and inoculated in a new culture vessel with…
A: Introduction Primary cell culture is the culture of cells directly isolated from parental tissue of…
Q: 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: 6)Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a)…
A: Answer. Staining is a biochemical technique of adding a class-specific dye to a substrate to…
Q: What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating")…
A: Quadrant streak method is used for isolating the bacteria based on their differences. Four Agar…
Q: 1. why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
A: Endospores are formed by certain gram positive bacteria such as species of Bacilli. These are highly…
Q: Although sparse, there is still visible growth of microorganisms in Plate D which is the…
A: Inoculation is defined as the act, process, or occurrence of inoculating, specifically from one…
Q: Some antibiotics show no zone of inhibition against the microbe. How is this possible? Explain how…
A: The zone of inhibition is basically the circular area around the spot of antibiotic in which the…
Q: 3. Based on his findings at the farm, the USDA inspector was also conçerned that the milk could be…
A: The E. coli O157:H7 is the bacterial species responsible for causing disease which typically…
Q: Placing bacteria onto the medium in a Petri dish is known as
A: Bacteria are the unicellular prokaryotic organism. Bacteria belongs to the kingdom Monera. Bacterias…
Q: 4. The Gram stain, if the decolorizing step is deleted, gram-negative cells will appear at the…
A: Decoloriser in gram staining method is to dehydrate the peptidoglycan layer of the cell wall causing…
Q: 1. Indicate the objective of this method. 2. Pick out the donor.. . of & the recipient.. 3. Why we…
A: Deoxyribonucleic acid (DNA) stores the cell’s genetic information and is present in the nucleus of…
Q: 6/ The critical process in microscopic preparation of samples is * Mounting O Labelling Staining 8/…
A: 6- The critical process in microscopic preparation of sample is staining. In staining process cells…
Q: 1. Differentiate the types of culture media based on its physical state and uses.
A: MICROBES They are unicellular organisms that cannot be seen with the naked eye.This is why they are…
Q: If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the…
A: Bacterial staining is a very important process to visualize the bacterial cell morphology. If there…
Q: 1. In the Microbiology lab, staining methods are employed to appreciate the a. Shape and arrangement…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: What are the basic differences from a primary cell culture, cell line and a cell strain? 2.…
A: Answer 1: - Primary cell culture is defined as: - an ex vivo culture of cells freshly obtained from…
Q: 12. A differential media a) allows visual distinction between bacteria to perform a specific…
A: The cells are classified as unicellular and multicellular. The unicellular cells are known as…
Q: Draw a typical bacterial growth curve. Label the stages of growth and the axis of the graph.
A: A typical bacterial growth curve contains 4 phases. 1.Lag phase 2.Log/Exponential phase…
Q: 5. Using a pencil as your loop draw the streaking pattern used on a Petri plate to best achieve…
A: In Microbiology streaking is one of the techniques to isolate a pure strain from a culture of…
Q: The colonies on a negative MSA plate would appear _____________. 2. "E. Coli and S. epidermidis…
A: MSA stands for Mannitol Salt Agar. The MSA plate is often used for halophilic bacterias. Halophilic…
Q: 6- The bacterial growth curve; O would have a shape which is dependent upon factors such as medium,…
A: The bacterial growth curve shows the number of live cells in a bacterial population over any period…
Q: What is the most common sterilization technique used in laboratories?
A: According to the guidelines we have to answer only first question, rest you can ask separately thank…
Q: 12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but…
A: A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when…
Q: 4. Which of the following stages is not performed while isolating a primary culture. O Isolation of…
A: Introduction: A primary culture is the stage of the culture following cell isolation but prior to…
Q: 4. A medium containing an antibiotic is a selective medium. True or False.
A: In the study of microorganisms, a variety of mediums are utilised. The following are the definitions…
Q: 1. Why is it helpful that the transmission compound light microscopes we studied in lab are both…
A: Introduction A microscope is a scientific tool that is used to study items that are too small for…
Q: Discuss the freeze-drying process in details.
A: The process that helps remove the amount of moisture, provide flexibility, and preserve any fruit or…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 2 images
- 1. The selective ingredient in mannitol salt agar is ______________, which selects for the growth of Staphylococcus organisms.The coagulase test is used primarily to differentiate Staphylococcusaureus froma. other staphylococci b. streptococci c. micrococci d. enterococciDiscuss Voges Proskauer test for listeria monocytogenes, give the principles, reagents used if there's any, procedures, and describe the result.
- Explain the rapid’l.mono test for Listeria monocytogenes. Give the principles, reagents used if any, procedures and decribe the result.How does one can differentiate staphylococcus aureus from streptococcus, with the test use, color, shape etc.Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media where Staphyloccocus epidermidis plated is still pink? The media used would be the Mannitol Salt Agar Medium.
- Fill out the data table attached below with regard to the medically significant bacteria. Attached beside is a sample data table for Staphylococcus aureus. Microorganism/Causative Agent: Salmonella TyphiFill out the data table attached below with regard to the medically significant bacteria and the diseases it cause. Attached beside is a sample data table for Staphylococcus aureus Microorganism/Causative Agent: Salmonella TyphiIs UV treament more or less effective than disinfection when used against Saphylococcus aureus?
- The organism tested on Blood Agar (plate shown furthest to the left) is: a. Micrococccus luteus b. Staphylococcus aureus. c. Staphylococcus epidermidis You know because of which specific features of the hemolytic reactionThe patient, who presented with complaints such as sore throat, fever, weakness, and swollen tonsils, was examined by an ear, nose and throat doctor. Having diagnosed the patient with Streptococcus pyogenes pharyngitis, the doctor requested a throat culture from the patient to confirm the accuracy of the diagnosis. a. In which environment does the Laborant develop Streptococcus pyogenes in the throat culture taken from the patient with a swab? Explain by explaining. b. Laborant is Strep in mixed culture, which is formed as a result of throat culture that he developed (cultivated). Using which methods to analyze and determine pyogenes phenotypically? c. Phenotypically detected Strep. The laboratory technician, who differentiates the pyogenes from other bacterial species in the environment, develops the bacteria in which specific environment using which method? Explain by explaining.Draw the reactions and the interpretation on the lysine iron agar (lia) test for Proteus vulgaris