4. In standard plate counts, plates that are 'countable' contain between to colonies/plate. What does CFU/mL stand for? What is the CFU/mL is a bacterial samples was diluted 1:10,000 times and plate counts averaged 56 colonies/plate? (0.1 mL was plated out/plate Express your answer in scientific notation. 6. 5.
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- PLEASE ANS ASAP 7. Typhoid fever is caused by a species of: a . Escherichia b. Campylobacter c. Shigella. d. Salmonella 8. At the end of the Gram -staining procedure , Gram -positive bacteria will be: a. blue to purple b . pink to red c. orange. d. green 9. Which one of the following types of culture media is selective and differential? a. Phenylethyl alcohol agar b . Blood agar c. Thayer-Martin agar d. MacConkey agar 10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a.…1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21
- 1. TRUE or FALSE. Relative to a 24 hour culture of endospore forming bacteria, a 72 hour culture would contain more endospores.Hi, I want to ask the question related to antibiotics resistance? The question is the non-existance of solidifying agent may produce a liquid like culture media. Name the method used to determine the Antibiotic Susceptibility test which uses the said media? Can explain answer? Thank you.2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)
- 1.Would a syringe filter with a pore size of 0.22 micrometers be able to completely remove all microorganisms including viruses? Explain why or why not? 2. Under what conditions is sterilization by filtration preferred over sterilization by heat? (short answer)1. Use the words presented in below to create a concept map illustrating the relationships among these key terms – refer to “Five I’s” by Cowan and Smith (2018) to be guided. inoculation identification biochemical tests streak plate isolation medium subculturing mixed culture incubation multiplication source of microbes pure culture inspection staining transport medium wire loop 2. What is the importance of employing aseptic techniques? Give an example of a situation in the laboratory that might happen when this method is not practiced.Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred
- There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes c) test tubes d) microscope slides 2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top. b. You notice flames coming from the tubing of your Bunsen burnerPLEASE answer the 2 with no much explanation Culture media is sterilized after the inoculation of microbes to ensure optimal growth of target microorganisms True False Microwave oven available in the laboratory can be used to heat culture media, as well as food for lunch. True False