2. Determine Michaelis-Menten parameters Vmáx and KM for the reaction [ urea,urease]* k2 k3 2NH3 + CO2 + urease -H,O urea + urease - The reaction rate depending on the concentration is given by the following table: Curea (kmol/m³) -ľ urea (kmol/m³ s) 0,200 1,08 0,020 0,55 0,010 0,38 0,005 0,20 0,002 0,09 (Use the 3 forms of linearization and the least squares method, compare the results and discuss which was the best fit and why.)

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2. Determine Michaelis-Menten parameters Vmáx
and KM for the reaction
k.
[urea.urease]*
k2
k;
+ 2NH3 + CO2 + urease
-H,O
urea + urease a
The reaction rate depending on the concentration is given by the following table:
Curea (kmol/m³)
-l' urea (kmol/m³ s)
0,200
1,08
0,020
0,55
0,010
0,38
0,005
0,20
0,002
0,09
|(Use the 3 forms of linearization and the least squares method, compare the results and
discuss which was the best fit and why.)
Transcribed Image Text:2. Determine Michaelis-Menten parameters Vmáx and KM for the reaction k. [urea.urease]* k2 k; + 2NH3 + CO2 + urease -H,O urea + urease a The reaction rate depending on the concentration is given by the following table: Curea (kmol/m³) -l' urea (kmol/m³ s) 0,200 1,08 0,020 0,55 0,010 0,38 0,005 0,20 0,002 0,09 |(Use the 3 forms of linearization and the least squares method, compare the results and discuss which was the best fit and why.)
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