2. Discuss the mechanism of ethidium bromide (EtBr) and why is it used in staining DNA during visualization.

Q: 1. What does PCR allow you to do with DNA?

A: The separated and identified PCR results are then used to characterize the STR region under…

Q: If you want to run 5 μl of a PCR sample on an agarose gel, how much 6X loading dye would you prep…

A: In this question, we are given volume of PCR reaction that is 5 ul. The concentration of loading dye…

Q: 2) Explain how the polymerase chain reaction (PCR) works and how this technique can be employed to:…

A: Technology is utilized in science, while science is used in technology. Both are vital to our…

Q: (iii) Explain why DNA in the presence of monovalent cations will have a high melting temperature…

A: Melting temperature Tm Tm is defined as the temperature at which 50 percent of the double-stranded…

Q: 1. Which of the following is the correct order for the steps in DNA extraction? I. Precipitation of…

A: Hi! Since you have posted multiple questions and have not mentioned which to answer, we are…

Q: 3. What is a restriction digest? What does it mean if you were given a precut DNA? 4. What is…

A: Restriction digest It is the process of cutting DNA molecules into smaller pieces with special…

Q: 4. What makes the genetic modification of corn and bacterial cells similar to each other?

A: 4th Answer

Q: 3. What is in the master mix and why do you need each component? 4. Why do you need to perform PCR…

A: Polymerase chain reaction (PCR) is a molecular biology technique that is used for amplifying the…

Q: 4) Consider the following double-stranded DNA molecule: Complementary Strand: ATGTGTAGTGCGAGTTGA…

A:

Q: 1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed…

A: As per the guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: 6. Two possible paint mutations are the substitution of lysine for leucine or the substitution of…

A: Every physiological characteristic or metabolism of our body mainly controlled by proteins. These…

Q: 9. Why does the lagging strand need Ligase? * O Polymerase runs 5' to 3'. This creates fragments and…

A: Replication is the process which is responsible for producing daughter DNA molecules from the…

Q: 7. Explain the principle of quality determination of DNA.

A: *NOTE: Kindly repost for other questions. Dear Student as per the guidelines we are supposed to…

Q: 4. Write the Edman degradation technique used for the sequencing of proteins

A: “Since you have asked multiple questions, we will solve the first question for you. If youwant any…

Q: Why were nuclei being unstained when you used eosin? explain briefly

A: Hematoxylin is a deep blue-purple stain that stains nucleic acids through a complicated, poorly…

Q: What is the difference between an antigen test and a PCR test?          a. What are the benefits…

A: PCR- PCR stands for polymerase chain reaction. Antigen test- Antigen tests are immuno-assays which…

Q: 3. Which of the following is false? a) Methyl, phosphoryl, adenyl, uridylyl and adenosine…

A: Enzymes are basically proteins that are intricately packed within with the help of intramolecular…

Q: 7. Which best describes molecule A? a. It is an insulin gene. b. It is recombinant DNA. c. It is a…

A: Bacteria also called microbes are prokaryotic organisms that are minute and observed with a…

Q: DNA nicking assay

A: The DNA nicking assay is a powerful tool that can be used to measure the antioxidant and prooxidant…

Q: 6. Why do you think the Ames test is preferable to the use of animal models to screen chemical…

A: Ames test is used to find whether a given chemical causes mutation in the DNA in the animal models.…

Q: . Illustrate the basic steps in DNA extraction

A: The genetic material that is densely packed inside the nucleus and chromosomes is known as DNA. The…

Q: 1.a How does a cell recognize which strand is the sense strand? b. How does the RNA polymerase…

A: One of the important process in central dogma. It is a process of making a RNA copy using DNA; it is…

Q: 2. How does the universality of the genetic code make the recombinant DNA technology possible?

A: A nucleic acid is a linear polymer of nucleotides that is a component of the cell's information…

Q: 6. You perform a restriction digest that should yield 1,400 bp, 1,080 bp, 1,000 bp, and 400 bp…

A: There are different biomolecules, including DNA, RNA, proteins, etc., are present, and these…

Q: 3. Primer Design You are analyzing the

A: Primer is a short stretch of oligonucleotide which is designed to copy the gene sequence of interest…

Q: 3. A student performs an experiment similar to the one performed by Luria-Delbruck. However, the…

A: Experiment 1 shows that mutation are spontaneous and arise during cell division. This is the reason…

Q: 1. What does PCR stand for? 2. Explain the main objective of the PCR technique.

A: PCR It is a technique used in molecular biology which was developed by Kary Mullis, an American…

Q: 6) In automated DNA sequencing a) Radio labelled DNTPS are used b) Radio labelled ddNTPs are used c)…

A: Automated DNA sequencing is used to generate the nucleotide sequence of a given DNA strand. The…

Q: 1. What is the role of salt and dishwashing liquid in the extraction/isolation of DNA from yeast and…

A: "DNA extraction  or DNA isolation" is a procedure or a method wherein  DNA is purified by physical…

Q: blueprint for life?"

A: TE tris-EDTA buffer is an type of biological buffer system that used during isolation of a DNA which…

Q: 5- Which statement is not true for agarose gel electrophoresis? a) DNA fragments are separated based…

A: The technique which is used for the separation of DNA fragments is called agarose gel…

Q: What extra step need to be taken after collecting samples of many viruses before PCR can be…

A: The PCR i.e. polymerase chain reaction is performed in 3 basic steps: Collection of samples…

Q: 9. Explain why DNA fragments move through a gel at different rates. (A2)

A: A technique called gel electrophoresis is used to separate DNA fragments (or other macromolecules,…

Q: 4. Draw the actual map of the PMBBS plasmid, following the style of the sample map shown in Figure…

A: Restriction enzymes:  Restriction enzymes are also called molecular scissors.  These are named so…

Q: 7. Why recombinant DNA is very useful in improving our health conditions? A. Human insulin can be…

A: Of the many advancements in biotechnology, invention of rDNA technology is most significant because…

Q: What is the name of the process by which bacteria pick up a different organism’s genetic material?

A: 4. A bacterium takes up a fragment of DNA circulating in its surroundings during transformation. A…

Q: 3. What are the last three of six nucleotides (bases) of the palindromic site for the enzyme Ndel if…

A: Restriction endonucleases (or restriction enzymes) are enzymes that identify specific sequences in…

Q: Explain 2 possible consequences of accidentally puncturing one of the wells in the agarose gel in an…

A: The chromosomes in our cell cores comprise of huge strands of DNA. These strands are awkward when…

Q: 1. Why were nuclei being unstained when you used eosin? 2. What is the relationship between…

A: Answer 1- Eosin is a group of fluorescent acidic compounds that reacts with and forms salts with…

Q: Outline the 7 steps of 1H NMR.

A: Nuclear magnetic resonance or NMR spectra tells the number of C and H atoms that are present in a…

Q: Fast fast fast

A: Introduction The cells are so minute structures that we cannot see them with unaided eyes, we…

Q: 7. Discuss the modes of actions by which the following three compounds disrupt DNA- associated…

A: Whether its a cancer cell trying to multiply inorder to generate more cancer cell or a pathogen…

Q: 2. If a selection assay be made to identify cells that have incorporated the recombinant DNA, what…

A:

Q: 1. How does real-time polymerase chain reaction work? 2. How is SYBR Green used in the experiment?…

A: Note: " According to the guidelines only one question is answered. Please ask other questions…

Q: 9. Sequencing In order to ensure a reliable result, arrange the following procedure with A being the…

A: Mentioned steps of the procedure are performed to analyze the number of shoots and roots of onion.…

Q: 1.) What are the different temperatures used in PCR (polymerase chain reaction)?   What happens at…

A: Polymerase Chain Reaction or PCR is a technique that used for making copies or amplify  small…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.