Answered: 6. Why do you think the Ames test is…

6. Why do you think the Ames test is preferable to the use of animal models to screen chemical compounds for mutagenicity?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section18.1: Key Dna Technologies For Making Genetically Altered Organisms

Problem 3SB: What information and materials are needed to amplify region of DNA using PCR?

See similar textbooks

Related questions

Q: 2) Explain how the polymerase chain reaction (PCR) works and how this technique can be employed to:…

A: Technology is utilized in science, while science is used in technology. Both are vital to our…

Q: How are Recombinant DNA formed?What is the difference between genetic modification and selective…

A: Recombinant DNA technology is a new approach to modify the genome of a host organism by joining two…

Q: 2. Discuss the use of transposons as mutagens in bacteria.

A: Transposable elements are segments of DNA that can move around the genome. Classification of…

Q: explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain…

A: PCR is the polymerase chain reaction that use to amplify the particular sequence of DNA and forming…

Q: 3. What is a restriction digest? What does it mean if you were given a precut DNA? 4. What is…

A: Restriction digest It is the process of cutting DNA molecules into smaller pieces with special…

Q: 3. What is in the master mix and why do you need each component? 4. Why do you need to perform PCR…

A: Polymerase chain reaction (PCR) is a molecular biology technique that is used for amplifying the…

Q: 3. The Watson-Crick model pictures DNA as as the step. spiral staircase with the as the handrails…

A: DNA has a double-stranded structure in which two strands run in an anti-parallel direction. DNA…

Q: Which of the following alternative steps cannot be employed in the DNA extraction because it would…

A: You have asked multiple questions. I will answer 1st question, as allowed by guidelines. Asked :…

Q: 3. Why will we put your cheek cells in the InstaGene solution? In other words, why do we want to…

A: DNA isolation is one of the crucial steps in molecular biology and should be performed with care and…

Q: 4. Assume that a new low-calorie sweetener is developed. The structure is novel and is tested with…

A: Any product that is intended for human consumption requires prior approval and usage recommendation…

Q: Explain why phenol:chloroform is used in the isolation of genomic DNA ( What is the end result and…

A: Extraction of genomic DNA is of main importance to study the genetic reasons behind diseases and for…

Q: Methods of gene therapy

A: Note: Since you have posted multiple questions so we will be solving the first question for you. As…

Q: 4. Give the importance of Genomics especially in human. What are the different techniques in…

A: A genome is an organism's whole collection of DNA. The genomic DNA sequence is held within an…

Q: 2. What are restriction digestion and gel electrophoresis used for in this cloning series of…

A: Restriction digestion technique is implicated in biotechnology or related field during cloning…

Q: 4. Write the Edman degradation technique used for the sequencing of proteins

A: “Since you have asked multiple questions, we will solve the first question for you. If youwant any…

Q: If you will use apple or strawberries instead of banana do you think the result would be the same in…

A: All living things pass hereditary information from one generation to another using DNA as the basic…

Q: 3. Give a schematic diagram of the different steps involved in PCR (Polymerised chain reaction) of a…

Q: What is the difference between an antigen test and a PCR test? a. What are the benefits…

A: PCR- PCR stands for polymerase chain reaction. Antigen test- Antigen tests are immuno-assays which…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: 7. Based on the information provided, how many "cuts" would you expected after digesting the ligated…

A: The digestion of DNA with particular restriction enzyme is very critical process during the…

Q: 1. Describe the mutation that created the Hbs allele: type of mutation, location of mutation on HbA…

A: HbS allele is a mutated version of HbA allele. This results in Sickle cell anemia in human harboring…

Q: DNA nicking assay

A: The DNA nicking assay is a powerful tool that can be used to measure the antioxidant and prooxidant…

Q: 2. The restriction enzymes Xhol and Sall cut their specific sequences as shown below: Xhol 5'-c…

A: Restriction enzyme are also referred as restriction endonuclease. They are considered as a protein…

Q: 5) Which of the following is not a component of a PCR reaction mixture? A) DNA template B)…

A: Polymerase chain reaction (PCR) is a widely used method in molecular biology that is used to make…

Q: 1. What does PCR stand for? 2. Explain the main objective of the PCR technique.

A: PCR It is a technique used in molecular biology which was developed by Kary Mullis, an American…

Q: 1. What is the role of salt and dishwashing liquid in the extraction/isolation of DNA from yeast and…

A: "DNA extraction or DNA isolation" is a procedure or a method wherein DNA is purified by physical…

Q: Describe the experiments done by Hershey and Chase That Verified That DNA was the genetic material.…

A: Introduction: Researchers determined that the element responsible for the transmission of features…

Q: 1) Recombinant DNA technology and selective breeding are 2 techniques that seek to produce desirable…

A: WHAT IS RECOMBINANT DNA TECHNOLOGY AND SELECTIVE BREEDING ? - Recombinant DNA technology simply…

Q: What are the different elements you would need if you wanted to perform CRISPR to edit a gene?

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: 1. Where is the cleavable blocker located on the modified nucleotides used in Illumina sequencing?

A: Illumina sequencing is developed by Solexa and was subsequently acquired by Illumina. This…

Q: 5. Describe the method of random mutagenesis using degenerate oligonucleotide primers 6. What are…

A: We are authorized to answer one question at a time since you have not mentioned which question you…

Q: 5. What are some PROS and Cons of CRISPR? List at least 3 of ceach Pros of CRISPR Cons of CRISPR 6.…

A: “CRISPR” (pronounced “crisper”) stands for Clustered Regularly Interspaced Short Palindromic…

Q: 1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at…

A: DNA (Deoxyribonucleic Acid) is the genetic material of all living organisms whether plant, animal,…

Q: 7) The Human Genome project cost billions of dollars to complete. What was it and do you think it…

A: For long, many people thought of the Human Genome Project as biology's "moon shot." The United…

Q: 1. What is mutation? Explain the random and site-directed mutagenesis methods .Which one is…

A: Since you have posted a question with multiple sub-parts, we will solve first three questions for…

Q: What extra step need to be taken after collecting samples of many viruses before PCR can be…

A: The PCR i.e. polymerase chain reaction is performed in 3 basic steps: Collection of samples…

Q: 1 DNA evidence analysis is an imperfect science. Provide two reasons why this may be the case.

A: DNA is the hereditary material present in organisms. It is important for our growth, reproduction,…

Q: What is the purpose of adding of (a) warm salt water, (b) detergent, and (c) alcohol on the…

A: Extraction of DNA from a banana involves mashing, filtration, precipitation and extraction.

Q: 1. What is a plasmid? How are they used in bioengineering?

A: Answer : plasmid is the molecule which is known as the extrachromosomal molecule that is present in…

Q: How is a randomized DNA library created? MARK ALL THAT APPLY. the use of Mn2+ rather than Mg2+ by…

A: DNA libraries are a collection of a large number of various genes of choice kept or stored together…

Q: 3- Which of the following is not a basic requirement of PCR? a-Taq DNA polymerase b-DNA Probes…

A: Polymerase Chain Reaction (PCR). Principle: When a double-stranded DNA molecule is heated to a high…

Q: 1. List all the differences between the DNA Polymerase Reaction and Polymerase Chain Reaction (PCR)?

A: The flow of genetic information from DNA to RNA, RNA to protein synthesis is called central dogma.…

Q: 1. What is DNA cloning of animals and plants? 2. What is the importance of DNA cloning? 3. Give…

A: 1. DNA cloning of animals and plants is a process of creating a genetically identical copy of an…

Q: WHY DO WE NEED GENETIC ENGINEERING?

A: Genetic engineering is a vast field that mainly involves using rDNA technology to genetically alter…

Q: 3. The Watson-Crick model pictures DNA as a as the step. spiral staircase with the as the handrails…

A: The double helical structure of DNA was first proposed by James Watson and Francis Crick. According…

Q: Who are the scientists that proved DNA is the transferrable genetic material and Discuss how they…

A: Introduction DNA was never thought to be the genetic material as it was so inert and lacking…

Q: Explain the importance of Isolating DNA from the cells of organisms.

A: DNA is the genetic material for most organisms. The methods used to isolate DNA are dependent on the…

Q: 2. If a selection assay be made to identify cells that have incorporated the recombinant DNA, what…

Q: 1.) What are the different temperatures used in PCR (polymerase chain reaction)? What happens at…

A: Polymerase Chain Reaction or PCR is a technique that used for making copies or amplify small…

Q: 5. Describe the method of random mutagenesis using degenerate oligonucleotide primers

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Question

6. Why do you think the Ames test is preferable to the use of animal models to screen chemical compounds
for mutagenicity?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

1. What is/are the purpose(s) of DNA extraction? 2. What is the function of each reagent used in the extraction of DNA from a banana? a. Saltwater b. Liquid dishwashing soap c. Cold isopropyl alcohol
1. How can site-specific recombination be used in recombinant DNA technology? Answer and explain comprehensively.
1. What is the purpose of adding of (a) warm salt water, (b) detergent, and (c) alcohol on the experiment of the extraction of DNA from a banana?
PCR can be used _______. a. to increase the number of specific DNA fragments b. in DNA fingerprinting c. to modify a human genome d. a and b are correct
The results of a paternity test using short tandem repeats are listed in the table below. Whos the daddy? How sure are you?
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
3. Describe the experiments done by Hershey and Chase That Verified That DNA was the genetic material. Make sure you also include their conclusions.
1. If you forgot to add nucleotides to your PCR master mix, which control reaction tube would have results that are different than they would be if you had prepared them correctly? 7. If you found that there was DNA amplification in your negative control tube, what could be an explanation for that result?
4. Which type of PCR (End-point PCR, RT-PCR, qPCR)is used for diagnosing Covid-19 patients? Why is this PCR procedure used?
1. What is the function of the DNA polymerase enzyme in the PCR? 2. What natural process is PCR based on?
4. How is a randomized DNA library created? MARK ALL THAT APPLY. the use of Mn2+ rather than Mg2+ by DNA Polymerase PCR with dideoxynucleotides Mutagenic PCR Lots of books Add more dNTPs than normal to a PCR reaction