2.) One experimental tool used in the biological research and in clinical settings is called Fluorescent Activated Cell Sorting (FACS) or cell cytometry. For example, a cell membrane permeable (or nonpolar) chemical called Hoescht 33342 dye will specifically label DNA. Interpret the graph (Figure 17-4) that illustrates normal cells that have been labeled with Hoescht 33342 dye. and assign which phase(s) of the cell cycle (G1, S, G2, or M) corresponds to the fluorescence intensity. Justify your answers. relative fluorescence per cell Figure 17-4 Analysis of Hoechst 33342 fluorescence in a population of cells sorted in a flow cytometer (Problem 17-15). number of cells
2.) One experimental tool used in the biological research and in clinical settings is called Fluorescent Activated Cell Sorting (FACS) or cell cytometry. For example, a cell membrane permeable (or nonpolar) chemical called Hoescht 33342 dye will specifically label DNA. Interpret the graph (Figure 17-4) that illustrates normal cells that have been labeled with Hoescht 33342 dye. and assign which phase(s) of the cell cycle (G1, S, G2, or M) corresponds to the fluorescence intensity. Justify your answers. relative fluorescence per cell Figure 17-4 Analysis of Hoechst 33342 fluorescence in a population of cells sorted in a flow cytometer (Problem 17-15). number of cells
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 19P: Figure 28.11 depicts the eukaryotic cell cycle. Many cell types “exit� the cell cycle and...
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