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- Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature:
- Oven sterilization of aqueous culture medium. Effect: Rationale:Oven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium, slant agar tube, and broth tube for further study and identification Question from the picture above (pure colonies white pinhead size) How many pure colonies of microorganisms are in the petri dish sample?
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- Which isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count? A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply. ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms? A. inoculum B. culture medium C. diluent D. microbial cultureMicrobiology 1. What is the difference between quadrant streak method A from method B? 2. Why do we use a pencil instead of a pen when labeling culture tubes or plates? Are there other alternatives in labeling?1. One gram of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From the last dilution made, 200 µL was plated onto an all-purpose medium. After incubation, 123 colonies were counted. What is the CFU/mL in the sludge? Note: 1 gram sludge is equal to 1 mL sludge.