23 Samples of five plant tissue discs were incubated in dilute sodium chloride solution at different temperatures. After 24 hours, it was found that the uptake of ions from the solutions was as shown in the table (arbitrary units). Comment on how absorption of sodium chloride occurs, giving your reasons. Sodium ions Chloride ions Tissue at 5°C 80 40 Tissue at 25 °c 160 80
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- A glucose solution is supplied in a repeated fed batch culture. The following parameters have been measured at t=2.5 hours; the system was under a quasi-steady state at this time. V = 1.0L, F = 0.22L/hr, S_0 = 98g/L, mu_m = 0.31/hr, K_s = 0.14 g-glucose/L, YM_X/S = 0.48 g-cells/g-glucose, Xt_0 = 33g (1) Calculate the value of V0 and S. (2) Calculate the value of Xt for t = 2.5hrs.r^2=6Dt , where D is the diffusion coefficient of thediffusing object and t is the time that the object is allowed to diffuse. If the diffusion coefficient, D, of a small protein is 5 x 10^-10 m2 s-1, how long (on average) does ittake for the protein to diffuse across a parasitic wasp that is 189 um long ?A new 850 cm^2 membrane is being tested for use in a hemodialysis device. During testing, a model fluid mixture is used that contains solutes with average radii of 1 nm. In one test, the concentration of the filtrate is found to be 2.17 g/L, while the concentration on the feed-side surface is 8.52 g/L. Prior experimentation showed that the mass transfer coefficient in the device was approximately 9 × 10-4 cm/s. A) If the total filtration flow rate is measured to be 0.56 cm^3/s, what is the solute concentration in the feed solution? B) Explain how you could determine the average membrane pore radius for this membrane using this information.
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?• Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method Observe these experiments in these links: https://www.youtube.com/watch?v=jTDATlaBV-o https://www.youtube.com/watch?v=k1O9jBHgsxs Estimates of water potential of various tissues have been obtained after equilibrating the tissues in solutions of different osmotic potentials. The principle depends upon finding the concentration of sucrose that would cause no change in weight of the tissue before and after an incubation period in the solution. This concentration may be assumed to have a water potential equal to that of the tissue.Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.
- Suppose like :- At 10 am you received a doctors order that you have to Infuse 1,000ml of PNSS in 10 hours to Mr Dela Cruz. What is the correct rate of this isotonic solution when the drop factor is 10 gtt = 1ml ? 15.73 qtts/min 31.249 qtts/min 49 qtts/min 18 qtts/min.In a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 b. Construct an appropriately labeled graph to illustrate the sample means of the two locations to within 95% confidence (i.e. sample mean + 2 SEM).For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.
- The toxic effect of Na+ ions can be avoided in some plant species by storing them in membrane-bound vacuoles within the cytosol. Calculate the maximum vacuolar-to-cytosolic [Na+] ratio that could be achieved by a Na+/H+ antiport mechanism in the vacuolar membrane if the cytosolic pH is 7.4 and the vacuolar pH is 5.6.A mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the stationary phase. The elution buffer had a pH of 7.5. Proteins were eluted by increasing the ionic strength of the buffer. Predict the order of elution of the following proteins:Pepsinogen, pI = 1Cytochrome c, pI = 10.6Myoglobin, pI = 6.8i. Explain the behaviour of the amino acids (Alanine, Lysine, aspartic acid) during the ion exchangeprocedure.ii. Explain the behaviour of the amino acids (Alanine, Lysine, aspartic acid) during TLC. How does thesolvent affect the rate of migration iii. How does the column chromatography the amino acids (Alanine, Lysine, aspartic acid) with fraction eluted with HCl and phosphate buffer have an effect in cellulose TLC and the actual TLC?