A mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the stationary phase. The elution buffer had a pH of 7.5. Proteins were eluted by increasing the ionic strength of the buffer. Predict the order of elution of the following proteins: Pepsinogen, pI = 1 Cytochrome c, pI = 10.6 Myoglobin, pI = 6.8
A mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the stationary phase. The elution buffer had a pH of 7.5. Proteins were eluted by increasing the ionic strength of the buffer. Predict the order of elution of the following proteins: Pepsinogen, pI = 1 Cytochrome c, pI = 10.6 Myoglobin, pI = 6.8
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.6P
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A mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the
Pepsinogen, pI = 1
Cytochrome c, pI = 10.6
Myoglobin, pI = 6.8
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