2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA Polymerase III, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. coli strain in which DNA Polymerase III was inactivated (all its enzymatic activities were non- functional) - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells without DNA Pol III differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA Polymerase
III, that are active during prokaryotic
strain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-
functional) - assuming that all the other enzymes involved in replication remained fully
functional, how would DNA replication in these mutant cells without DNA Pol III differ from
DNA replication in normal E. coli? Briefly explain why you would expect to see that
change/those changes in DNA replication in the mutant cells.
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