2a. First, use your calculator to calculate the log of the molecular weight for each ladder band. (The first two were done for you.) Table 1: SDS PAGE Ladder Rf 0.04 0.205 0.27 0.3475 0.4475 0.545 0.6175 0.7575 0.8675 Molecular Weight (kDa) 188 62 49 38 28 18 14 a 3 Log (Molecular Weight) 2.27 1.79
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- You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.The main constituents in vinegar are water and ethanoic acid (CH3COOH). In order to determine the concentration of acid in homemade vinegar, a student titrated 25 cm3 of 001 M NaOH against the vinegar. The equation for the reaction is: CH3COOH(aq) + NaOH(aq) ® CH3COONa(aq) + H2O(l) The following titration results were obtained: Burette readings (cm3) Rough 1 2 Final burette reading 20.10 38.90 31.40 Initial burette reading 0.10 20.00 12.50 Volume of vinegar used 20.00 18.90 18.90 (a) What volume of vinegar should be used in the calculation? (b) What is the mole ratio of NaOH:CH3COOH? (c) Calculate the number of moles of alkali in 25 cm3 of NaOH solution used. (d) How many moles of acid were used in the titration? (e) Calculate the…Which one is the incorrect statement regarding mass spectrum? options: The (M)+• peak may be weak or entirely absent if it is particularly susceptible to fragmentation. The tallest peak in a mass spectrum is assigned a relative value of 100% and is called the base peak which always represent molecular ion, (M)+• According to nitrogen rule, an odd molecular weight indicates an odd number of nitrogen atoms, while an even molecular weight indicates either the absence of nitrogen or an even number of nitrogen atoms Mass spectrum shows the relative abundance of each cation detected The (M)+• peak indicates the molecular weight of the molecule
- What type of mass spectrometer can determine the structure and molecular weight of a compound like quercetin? Explain the principle behind this MS.f you were asked to write the chemical formula for one of the compounds in Model 1, which type of the drawing would be the easiest to use? Justify your reasoning.9. which of the following is an example of quantitive data? A)the freshman class of the students contained 643 students, 372 males, and 271 females. B)the absorption peak of cobalt chloride was 510nm C)bromelain has optimal enzyme activity at a pH of 7 D)the color of cobalt chloride was pink C) the mass of the onion was 50 grams 34. which of the following parts of the spectrophotometer covents light energy into electrical energy. 1. photometric energy 2. light energy 3. monochromator 4. phototube 5. sample holder
- 3. a) An aqueous solution is 3.50% by mass potassium bromide, KBr, and has a density of 1.02 g/mL. The molality of potassium bromide in the solution is _________m. b)An aqueous solution of magnesium nitrate has a concentration of 0.474 molal. The percent by mass of magnesium nitrate in the solution is _________%. c)An aqueous solution of cobalt(II) bromide has a concentration of 0.153 molal. The percent by mass of cobalt(II) bromide in the solution is _________%.Answer D E and F d) in base pairing, why do we never see AG, GA, CT or TC forming complementary base pairs? e) why is vegetarianism a more energy intensive process (from the organism standpoint) than being omnivores? [DON'T EVEN THINK OF STATING ANYTHING ABOUT FIBER, BULK, AND/OR CELULOSE!!!!] f) why is significant serum (in the bloodstream) cholesterol reduction so difficult, and why do most of the drugs designed to reduce cholesterol target the liver?Using the equation for the best-fit straight line through your data, the average absorbance of your unknown samples U1 and U2, and any dilution factors (DON’T LEAVE OUT THE DILUTION FACTOR), calculate the concentration of protein in the original unknown protein sample. y = 1.6849x + 0.0414R² = 0.9904
- Expanding what we know. In the last example in the video, the empirical mass was 101.9. What if instead of the given molecular mass, I'd told you that the molecular as was 305.7, what would the molecular formula be. (Be sure to do it in order C#Cl#F# so that the program will accept your answer)Specific rotation of L-alanine [(+)alanine) in water (at 25 °C) is +2.8. Suppose you have 2.00 g of mixture of L and D-alanine [(-)alanine]. You dissolved it in 10 mL of water, and measured rotation in 10 cm cell. The observed rotation was +0.12. Calculate the ee% for the mixture. How many grams of D- and L-alanine do you have in the sample?31. The formula for a cosmetic cream calls for 5% of an emulsifier blend consisting of Span 60 and Tween20. If the Required HLB of the oil phase is 14.0, how much of each of the emulsifiers, respectively, shouldbe used in preparing 500 grams of the cream? (HLB for Span 60 = 4.7; HLB for Tween 20 = 16.7)a. 5.6 grams; 19.4 gramsb. 8.4 grams; 16.6 gramsc. 12.5 grams; 12.5 gramsd. 15.2 grams; 9.8 grams