3) Analysis of the sequence of a DNA fragment shows the presence of restriction site for the enzyme Hpall. However, the enzyme is not cutting at the site to generate expected fragment cleavage. What may the reason(s)?

Biology: The Dynamic Science (MindTap Course List)
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Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
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Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
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3) Analysis of the sequence of a DNA fragment shows the presence of restriction site for
the enzyme Hpall. However, the enzyme is not cutting at the site to generate expected
fragment cleavage. What may the reason(s)?
Transcribed Image Text:3) Analysis of the sequence of a DNA fragment shows the presence of restriction site for the enzyme Hpall. However, the enzyme is not cutting at the site to generate expected fragment cleavage. What may the reason(s)?
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