The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the expected number of NotI cleavage sites in the bacteriophage l genome, a linear DNA duplex 48.5 kbp in length with a (G + C) content of 50%.

Human Anatomy & Physiology (11th Edition)
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The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the expected number of NotI cleavage sites in the bacteriophage l genome, a linear DNA duplex 48.5 kbp in length with a (G + C) content of 50%.

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Concept Introduction

Restriction endonucleases are enzymes that cut DNA sequences at a specific site known as a restriction site. It is present in prokaryotes that exerts protection against invading bacteriophages.

The type I restriction enzymes cleaves at random sites that are greater than 1000 bp distant from the recognized restriction site. Type II restriction enzyme recognizes and cleaves within the restriction site.

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