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- Radiolabeling with 14C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at Cy with 14C.The acyl-CoA dehydrogenase deficiency diseases are a groupof inherited defects that impair the b-oxidation of fatty acids.Symptoms of the disease range from nausea and vomiting tofrequent comas. Symptoms may be alleviated by eating regularly and avoiding periods of starvation (12 hours or more).Why does this simple procedure alleviate the symptoms?A patient who has been drinking large amounts of alcohol for long periods of time shows thefollowing symptoms: apathy, loss of memory, and a rhythmical to-and-fro motion of the eyeballs.Which of the following reactions are most likely to be affected in the patient? A. Conversation of pyruvate to acetyl-CoA B. Conversation of a-ketoglutarate to succinyl-CoA C. Both A and B D. Neither A nor B
- Consider the docosanoic acid, C21H43CO2H a. Label the a and B carbons b. Draw the acyl CoA derived from this fatty acid c. How many acetyl CoA molecules are formed by complete B-oxidation? d. How many cycles of B-oxidation are needed for complete oxidation? e. How many molecules of ATP are formed from the complete catabolism of this fatty acid?1. Select the INCORRECT statement about Glutamate dehydrogenase : a. Catalyzes the removal of NH4+ into glutamate b.Catalyzes the incorporation of NH*4 into a-ketoglutarate c. Catalyzes the removal of NH*4 from glutamate C d. Catalyzes the incorporation of NH'4 into a-keto acidFive coenzymes are required by a-ketoglutarate dehydrogenase, the enzyme in the citric acid cycle that converts a-ketoglutarate to succinyl-CoA.a. Identify the coenzymes.b. Propose a mechanism for the reaction. A-ketoglutarate dehydrogenase A-ketoglutarate succinyl-CoAOO OO O−O O− −O SCoA + CO2
- Complete the table below. Consider docosanoic acid (C21H43CO2H) Questions Solutions Answers a. Label the alpha and beta carbons. b. Draw the acetyl CoA derived from this fatty acid. c. How many acetyl CoA molecules are formed by complete B-oxidation? d. How many cycles of B-oxidation are needed for complete oxidation? e. How many molecules of ATP are formed from the complete catabolism of this fatty acid?Upon considering the complete oxidation of one mole of fatty acyl (CoA) of an arachidic acid (20:0). Answer the following lettersa. Rounds of beta oxidationb. total no. of acety CoA producedc. total no. of NADH produced from all rounds of beta oxidariond. total no. of FADH2 produced from all rounds of beta oxidationWhich of the following citric acid cycle enzymes catalyzes substrate-level phosphorylation? A) isocitrate dehydrogenase B) α-ketoglutarate dehydrogenase C) succinyl CoA synthetase D) succinate dehydrogenase E) malate dehydrogenase
- For myristic acid, C 13H 27CO 2H: (a) How many molecules of acetyl CoA are formed from complete β-oxidation? (b) How many cycles of β-oxidation are needed for complete oxidation?CHOOSE THE CORRECT LETTER 1.Which of the following substrates is used in the first oxidation step of the Kreb's cycle to produce NAH and CO2?A. isocitrateB.a-ketoglutarateC. citrateD. succinyl-CoA 2.What is the reason for isomerization of citrate to isocitrate?A.The reaction converts a tertiary alcohol, which cannot easily be oxidized, to a secondary alcohol that can be oxidizedB. Isocitrate protects cells from the toxic effects of arsenite ion.C. The reaction is one major regulatory step for the citric acid cycle because it functions as a rate limiting step.D. Isocitrate is a substrate for a reaction that occurs spontaneously without enzymatic catalysisIf 14CO2 (radioactive carbon) were incorporated into the TCA cycle via the Pyruvate Carboxylase reaction which of the following molecules would contain radioactive carbon? a. Oxaloacetate b. Citric Acid c. Isocitrate d. Alpha-ketoglutarate e. Succinyl-CoA f .Succinate g. Fumarate h. Malate