3) Prepare 250 ml of 0.1 M Tris-HCI buffer, pH 7.8 as follows: Weigh 3.029 g of Tris = Tris (hydroxymethyl) aminomethane, m.wt. 121.14 Add 2.78 ml of 6N HCI pK of Tris = 8.1 SHOW CALCULATIONS IN YOUR LAB. REPORT For the assay, measure 50 ml of each buffer into two 100 ml beakers. Use a clean 50 ml cylinder for each buffer.
3) Prepare 250 ml of 0.1 M Tris-HCI buffer, pH 7.8 as follows: Weigh 3.029 g of Tris = Tris (hydroxymethyl) aminomethane, m.wt. 121.14 Add 2.78 ml of 6N HCI pK of Tris = 8.1 SHOW CALCULATIONS IN YOUR LAB. REPORT For the assay, measure 50 ml of each buffer into two 100 ml beakers. Use a clean 50 ml cylinder for each buffer.
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter14: Equilibria In Acid-base Solutions
Section: Chapter Questions
Problem 71QAP: The species called glacial acetic acid is 98% acetic acid by mass (d=1.0542g/mL). What volume of...
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