3. A mixture of proteins will be separated on a gel filtration column. The first peak elutes in 12 ml from the column, and the last peak elutes in 42 ml. The flow rate in the column is 1.5 ml/minute. The column diameter is 1.0 cm, and the height is 60 cm. Protein MW Кр 1 NaCl 58.44 aprotinin 6500 0.69 43,000 75,000 2,000,000 ovalbumin 0.43 Conalbumin 0.35 Blue Dextran

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter14: Formula Method
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3. A mixture of proteins will be separated on a gel filtration column. The first peak elutes
in 12 ml from the column, and the last peak elutes in 42 ml. The flow rate in the column is 1.5
ml/minute.
The column diameter is 1.0 cm, and the height is 60 cm.
Protein
MW
Кр
NaCl
58.44
1
aprotinin
6500
0.69
43,000
75,000
2,000,000
ovalbumin
0.43
Conalbumin
0.35
Blue Dextran
a. Calculate the elution time of each protein.
b. Explain the assumptions that can be used to scale up the column to a flow rate of 200
ml/minute.
c. Calculate the dimensions of the new column.
||
Transcribed Image Text:3. A mixture of proteins will be separated on a gel filtration column. The first peak elutes in 12 ml from the column, and the last peak elutes in 42 ml. The flow rate in the column is 1.5 ml/minute. The column diameter is 1.0 cm, and the height is 60 cm. Protein MW Кр NaCl 58.44 1 aprotinin 6500 0.69 43,000 75,000 2,000,000 ovalbumin 0.43 Conalbumin 0.35 Blue Dextran a. Calculate the elution time of each protein. b. Explain the assumptions that can be used to scale up the column to a flow rate of 200 ml/minute. c. Calculate the dimensions of the new column. ||
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