CFURemember:= Number of Colonies Counted xml.1Dilution Factor# of ml. plated outTreat grams dirt or organic material as follows: 1 gram1 cubic centimeter (1 cm) =1 mL.%3DCFUV;D;=V,D2OCD =Volume x DilutionV=VolumeD=dilution1. Calculate the CFU/ml of a urine sample if 49 E. coli colonies were counted on NA when 50microliters were plated on the NA plate from a 10-6 dilution tube.2. Plating 0.1 mL of a sample diluted by a factor of 10 produced 43 colonies. What was theoriginal concentration (CFU/ml) in the sample?3. You have inoculated 100 microliters of a sample diluted by a factor of 103 on a NA plate.After incubation, you count 58 colonies. What was the original concentration (CFU/ml) inthe sample?

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1. Calculate the CFU/ml of a urine sample if 49 E. coli colonies were counted on NA when 50 microliters were plated on the NA plate from a 10 dilution tube.

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter15: Fluid And Acid-base Balance

Section: Chapter Questions

Problem 3SQE

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Question

CFU
Remember:
= Number of Colonies Counted x
ml.
1
Dilution Factor
# of ml. plated out
Treat grams dirt or organic material as follows: 1 gram
1 cubic centimeter (1 cm) =1 mL.
%3D
CFU
V;D;=V,D2
OCD =
Volume x Dilution
V=Volume
D=dilution
1. Calculate the CFU/ml of a urine sample if 49 E. coli colonies were counted on NA when 50
microliters were plated on the NA plate from a 10-6 dilution tube.
2. Plating 0.1 mL of a sample diluted by a factor of 10 produced 43 colonies. What was the
original concentration (CFU/ml) in the sample?
3. You have inoculated 100 microliters of a sample diluted by a factor of 103 on a NA plate.
After incubation, you count 58 colonies. What was the original concentration (CFU/ml) in
the sample?

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