4) A DNA sample was obtained from a new species of a rabbit. For further investigations, 5.0 mL of the DNA sample was obtained and diluted to 12.5 mL using 2.0 M phosphate buffer pH 7.2. Afterwards, 2.0 mL of this solution was obtained and diluted with 5.0 mL of the same buffer. Finally, a 1:4 dilution was prepared from the diluted solution. The final concentration of the diluted DNA solution was found to be 5.27 µg/L.
4) A DNA sample was obtained from a new species of a rabbit. For further investigations, 5.0 mL of the DNA sample was obtained and diluted to 12.5 mL using 2.0 M phosphate buffer pH 7.2. Afterwards, 2.0 mL of this solution was obtained and diluted with 5.0 mL of the same buffer. Finally, a 1:4 dilution was prepared from the diluted solution. The final concentration of the diluted DNA solution was found to be 5.27 µg/L.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:4) A DNA sample was obtained from a new species of a rabbit. For further
investigations, 5.0 mL of the DNA sample was obtained and diluted to 12.5 mL
using 2.0 M phosphate buffer pH 7.2. Afterwards, 2.0 mL of this solution was
obtained and diluted with 5.0 mL of the same buffer. Finally, a 1:4 dilution was
prepared from the diluted solution. The final concentration of the diluted DNA
solution was found to be 5.27 ug/L.
a. What is the dilution factor?
b. What is the actual concentration of the DNA from the new rabbit
species?
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