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- The Rf of the major compound in the TLC shown below isPlease find the absorbance and plot te calibration curveSay you’ve just ran your unknown sample on the uv/vis and realized theabsorption is greater than the largest absorption of your standard curve. This is bad from a quantitative standpoint, as we can’t compare our standard curve with our data. What would you need to do to fix the problem? (Hint: there are two possible answers for this.)
- A scanner tube is used in STM to precisely control the tip/sample position. What materialis in general the scanner tube made of and what is its working mechanism?Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.Explain if we need or not a quartz cuvet when doina UV-visible Absorption spectrometry.
- The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.Explain how beam chopping reduces line noise and noise from source drift.If you goop on more sunscreen to double its thickness, absorbance will be doubled. What will be the transmittance near 300 nm and what percentage of ultraviolet radiation is blocked?
- What should you put into the reference cuvet when doing UV-visible Absorption spectrometry.fill in the column thats empty by finding the standard deviation for each average absorbance, with work please.What percent transmittance range and what absorbance range are considered to be the optimum working ranges for spectrochemical measurements?