Prepare a standard curve from the data below from a Bradford assay. Samples were prepared from a 0.100 mg/mL egg white lysozyme stock solution in a 100.0 mM, pH 7.0 phosphate buffer at various dilutions in a final volume of 1.0 mL. The data is shown in the table below: Dilution (%) 0.0 (blank) 2.5 → 0.0025 mg 5.0 → 0.005 mg Absorbance @ 595 nm (1 cm pathlength) 0.000 0.031 0.075 10.0 → 0.01 mg 0.134 15.0 → 0.015 0.229 20.0 → 0.02 0.280 25.0 → 0.025 0.386 37.5 → 0.0375 0.540 50.0 → 0.05 mg 0.742

Prepare a standard curve from the data below from a Bradford assay. Samples were prepared from a 0.100 mg/mL egg white lysozyme stock solution in a 100.0 mM, pH 7.0 phosphate buffer at various dilutions in a final volume of 1.0 mL. The data is shown in the table below: Dilution (%) 0.0 (blank) 2.5 → 0.0025 mg 5.0 → 0.005 mg Absorbance @ 595 nm (1 cm pathlength) 0.000 0.031 0.075 10.0 → 0.01 mg 0.134 15.0 → 0.015 0.229 20.0 → 0.02 0.280 25.0 → 0.025 0.386 37.5 → 0.0375 0.540 50.0 → 0.05 mg 0.742

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter14: Chromatography

Section: Chapter Questions

Problem 1P

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A 10 ml of ascorbic acid (MW=176.1) injection was transferred to a 100 ml volumetric flask and made up to volume with deionized water. A 20 ml of the diluted injection was transferred to a conical flask and titrated with Iodine VS using starch mucilage as the indicator. The iodine solution was standardized against a solution of sodium thiosulfate primary standard (MW=248.2). Results: Weight of sodium thiosulfate used to prepare 100 mL primary standard solution = 2.501 g Volume of thiosulfate solution required to titrate 25 mL of Iodine solution = 24.83 mL Volume of iodine solution required to titrate 20 mL of the diluted ascorbic acid injection = 21.55 mL Stated content of the injection = 10% w/v Calculate the % of the stated content in the injection. (Round-off your answer to 2-decimal places) Answer = ____%
A 10 ml of ascorbic acid (MW=176.1) injection was transferred to a 100 ml volumetric flask and made up to volume with deionized water. A 20 ml of the diluted injection was transferred to a conical flask and titrated with Iodine VS using starch mucilage as the indicator. The iodine solution was standardized against a solution of sodium thiosulfate primary standard (MW=248.2). Results: Weight of sodium thiosulfate used to prepare 100 mL primary standard solution = 2.501 g Volume of thiosulfate solution required to titrate 25 mL of Iodine solution = 24.83 mL Volume of iodine solution required to titrate 20 mL of the diluted ascorbic acid injection = 21.55 mL Stated content of the injection = 10% w/v Calculate the % of the stated content in the injection.
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In a GC run, dodecane was used as test solute for a 30 m x 250 ?m i.d. DB-5 column. The dodecane peak eluted at 5.2 min. and the distance between its two inflection points was 4.0 s. This was the number of theoretical plates generated by this column:
I have set up my spreadsheet and was able to reproduce the result with a pKa of 4.64. TRUE OR FALSE