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Explain if we need or not a quartz cuvet when doina UV-visible Absorption spectrometry.
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- What is laser ablation and how can it he used to sample solids for ICPMS?What should you put into the reference cuvet when doing UV-visible Absorption spectrometry.Why is it important to acquire a reference spectrum and subtract it from the sample’s spectrum? Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)? What would happen if you put too much of a compound in the cuvette during the spectral measurement? Why is it important to avoid any scratch on the cuvette? Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not?
- Analyze the effect of concentration and path length on the absorption ofradiation in UV visible spectrophotometer.Give one advantage of the proposed HPLC identification test over the current UV-Visible Spectroscopy method of Amiloride Hydrochloride.What are the important diagnostic peaks in this IR spectra? Fingerprint region <1500cm^-1 is not necessary. Thank you.
- Does a calibration curve alwasy compare concentration and absorbance? Or can it compare concentration and %T as well?How a UV-Vis spectrophotometer can be used in analyzing a colorless sample? Please answer in detail and clearly. Thank you so much!Why acid is added to the solution when analyzing mixtures in the UV-Visible absorption spectrometry.
- When working with Atomic Absorption Spectrophotometer, tell the criteria to work with which flame and in which part of the flame?Calculate the Absorbance from concentration for each trial of 0,10, 30, 40, 50 % (with yellow LED) Absorbance=−log(Percent Transmittance/100) - did my best with transmission ruler, do the numbers look rightGiven the background IR spectra, rank the three methods (Nujol mull, CCl4 solution, and KBr pellet) in order of best to worst spectra collected on a solid sample and explain your ranking.