4. Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene Х. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of , Х. gene Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e., it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA, RNA, and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X, its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. The positions of the wild type bands are indicated. 3 points: +1 point for each completely correct blot. 1 2 3 4 1 2 3 4 1 3 4 Southern Blot Northern Blot Western Blot

4. Suppose you are studying gene X in yeast using four different strains. Strain 1 is haploid and has the wild type allele of gene X. Strain 2 is haploid and has a missense mutation halfway through the coding sequence of gene Х. Strain 3 is haploid and has a nonsense mutation halfway through the coding sequence of , Х. gene Strain 4 is diploid and is heterozygous for the nonsense mutation in gene X (i.e., it carries the wild type allele of gene X on one chromosome and the nonsense allele of gene X on the other homologue). You have a DNA probe that will hybridize with gene X and its mRNA transcript on southern and northern blots, respectively. You also have an antibody that will bind to the protein encoded by gene X on western blots. You extract DNA, RNA, and protein from the four strains described above and do southern, northern, and western blot analyses to compare gene X, its mRNA transcript, and its protein in these strains. Please draw the bands you expect to see. The positions of the wild type bands are indicated. 3 points: +1 point for each completely correct blot. 1 2 3 4 1 2 3 4 1 3 4 Southern Blot Northern Blot Western Blot

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 3TYK

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