4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of 400 µL in each well. Calculate the volumes of each reagent that needs to be added and complete the table below (fill in the blue boxes). [BSA] µg/ BSA (µL) Bradford Total volume Absorbance Well Water (µL) Reagent (µL) OD 595nm (A595) (µL) A1 100 200 400 A2 1 99 200 400 0.05 АЗ 2 200 400 0.10 A4 4 4 200 400 0.25 A5 6. 6. 200 400 0.3 A6 8 200 400 0.38 A7 10 200 400 0.55 A8 20 200 400 0.77 Table 2: Calculation of volumes to add for experiment
4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of 400 µL in each well. Calculate the volumes of each reagent that needs to be added and complete the table below (fill in the blue boxes). [BSA] µg/ BSA (µL) Bradford Total volume Absorbance Well Water (µL) Reagent (µL) OD 595nm (A595) (µL) A1 100 200 400 A2 1 99 200 400 0.05 АЗ 2 200 400 0.10 A4 4 4 200 400 0.25 A5 6. 6. 200 400 0.3 A6 8 200 400 0.38 A7 10 200 400 0.55 A8 20 200 400 0.77 Table 2: Calculation of volumes to add for experiment
Introductory Chemistry: A Foundation
9th Edition
ISBN:9781337399425
Author:Steven S. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Donald J. DeCoste
Chapter15: Solutions
Section: Chapter Questions
Problem 130AP
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