(4521) Ahdl (3628) Peit (1001) Scal (4801) Pul 4000 (3249) Bt2171 (3197) Bam (5325) Sep 5000 Ampl (3052) Pol SV40 poly(A) signal (2959) BB (5442) gr O streptavidin column O anti-myc column o hydrophobic column ○ Ni²+-NTA column AmpR promoter 3000 Bill (12) (2793) R 5444 bp NeoR/Kank Mel (161) Bpu101 (180) Nrul (20) Mul (228) Spel (249) Ndel (44) CMV enhancer CMV promoter BHII (2674) SV40 on 17 prom er | MCS 5GH poly(A) signa O OPAS 0002 Snabl (590) for Nhel (95) Bmt (199) Kast (2276) Pmel (904) ANI (908) Hind SexAI (1834) BRI (2063) Stul (2066) Avril (2067) TspMI-Xmal (2008) Smal (2090) BsaB (2136) Nart (2277) Stol (2278) PluT! (2290) Mack (2359) PAFI Th1111 (295) (911) Berest (17) (921) Kont BamHI (29) EcoRI (935) EcoRV (147) BEXI (957) Not! (962) PaeR71 -PspXI-Xhol (965 Xbal (974) Eco01091 PspOMI (960) Apal (984) Myc Bbs (1230) Drall (1544)

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
Problem 3RQ
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What column can be used to purify a protein produced using the vector below?
(4521) AhdI
(3628) Pil
(5001) Scal
(4891) Pul
£4000
(3249) BstZ171
(3197) Baml
(5325) Sept
5000
AmpR
(3012) Pol
(5442) SgrDI
SV40 poly(A) signal
(2959) BIBI
O streptavidin column
O anti-myc column
O hydrophobic column
○ Ni²+-NTA column
IK
AmpR promoter
3000
Ball (12)
(2793) Rsrl
5444 bp
Mel (161)
Bpu101 (180)
Nrul (20)
Mlul (228)
CMV
NeoR/Kank
chancer Sar
CMV promromot
BsSHII (2674)
ear M
SV40 or
n
n
24
Spel (249)
Nel (484)
17)
1000
bGH po
promote
SnaBI (590)
Nhel (895)
Bmtl (899)
poly(A) signal
C0027
ori
PluTI (2290)
MsCI (2359)
PRIFI- Th1111 (2395)
Created with SnapGene
Pmel (904)
AMII (908)
HindIII (911)
Arch11 (917)
Kpnl (921)
BamHI (929)
EcoRI (935)
EcoRV
(947)
BetXI (957)
Not! (962)
Kast (2276)
Nart (2277)
Stol (2278)
PaeR71 - PspXI - XhoI (968)
PspOMI (960)
Xbal (974)
Eco01091
Apal (984)
Myc
Bbs (1230)
Drall (1544)
-BseRI (2053)
Stul (2066)
AvrII (2067)
TspMI Xmal (2008)
Smal (2090)
BsaB1 (2136)
SexAI (1834)
Transcribed Image Text:What column can be used to purify a protein produced using the vector below? (4521) AhdI (3628) Pil (5001) Scal (4891) Pul £4000 (3249) BstZ171 (3197) Baml (5325) Sept 5000 AmpR (3012) Pol (5442) SgrDI SV40 poly(A) signal (2959) BIBI O streptavidin column O anti-myc column O hydrophobic column ○ Ni²+-NTA column IK AmpR promoter 3000 Ball (12) (2793) Rsrl 5444 bp Mel (161) Bpu101 (180) Nrul (20) Mlul (228) CMV NeoR/Kank chancer Sar CMV promromot BsSHII (2674) ear M SV40 or n n 24 Spel (249) Nel (484) 17) 1000 bGH po promote SnaBI (590) Nhel (895) Bmtl (899) poly(A) signal C0027 ori PluTI (2290) MsCI (2359) PRIFI- Th1111 (2395) Created with SnapGene Pmel (904) AMII (908) HindIII (911) Arch11 (917) Kpnl (921) BamHI (929) EcoRI (935) EcoRV (947) BetXI (957) Not! (962) Kast (2276) Nart (2277) Stol (2278) PaeR71 - PspXI - XhoI (968) PspOMI (960) Xbal (974) Eco01091 Apal (984) Myc Bbs (1230) Drall (1544) -BseRI (2053) Stul (2066) AvrII (2067) TspMI Xmal (2008) Smal (2090) BsaB1 (2136) SexAI (1834)
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