Question 2. You have a wild-type strain of E. coli with the genotype A B C D E F You introduce an F+ plasmid into your wild-type strain and isolated a few Hfr derivative strains that you call Hfr1, Hfr2, and Hfr3. You are studying several new genes in E. coli with interesting phenotypes. You obtain a multiply mutant strain with chromosomal genotype: a b c d e f a) You mate each Hfr strain to your multiply-mutant strain in a separate experiment. At various times you interrupt the matings and plate the bacteria under conditions in which only the recipient strain can grow. You obtain the following earliest-time-of-entry data, in minutes: Gene Hfr1 Hfr2 Hfr3 A B D E F - 11 11 13 7 26 - 9 16 5 31 15 27 31 11 Draw a map of these genes that is consistent with the data, including all the genes and Hfr origins, the distances between them (in minutes), and the direction of transfer of each Hfr.
Question 2. You have a wild-type strain of E. coli with the genotype A B C D E F You introduce an F+ plasmid into your wild-type strain and isolated a few Hfr derivative strains that you call Hfr1, Hfr2, and Hfr3. You are studying several new genes in E. coli with interesting phenotypes. You obtain a multiply mutant strain with chromosomal genotype: a b c d e f a) You mate each Hfr strain to your multiply-mutant strain in a separate experiment. At various times you interrupt the matings and plate the bacteria under conditions in which only the recipient strain can grow. You obtain the following earliest-time-of-entry data, in minutes: Gene Hfr1 Hfr2 Hfr3 A B D E F - 11 11 13 7 26 - 9 16 5 31 15 27 31 11 Draw a map of these genes that is consistent with the data, including all the genes and Hfr origins, the distances between them (in minutes), and the direction of transfer of each Hfr.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:Question 2. You have a wild-type strain of E. coli with the genotype
A B C D EF
You introduce an F+ plasmid into your wild-type strain and isolated a few Hfr derivative strains that you
call Hfr1, Hfr2, and Hfr3. You are studying several new genes in E. coli with interesting phenotypes. You
obtain a multiply mutant strain with chromosomal genotype:
a b c d e f
a) You mate each Hfr strain to your multiply-mutant strain in a separate experiment. At various times
you interrupt the matings and plate the bacteria under conditions in which only the recipient strain can
grow. You obtain the following earliest-time-of-entry data, in minutes:
Gene Hfr1 Hfr2 Hfr3
A
B
C
D
E
F
11
13
7
I
26 16
11
9
5
31
15 27
31 11
Draw a map of these genes that is consistent with the data, including all the genes and Hfr origins, the
distances between them (in minutes), and the direction of transfer of each Hfr.
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