4mL of diluted protein solution was prepared by mixing 200uL of the protein solution with 3.8mL of distilled water. 100uL of this diluted was then mixed with 900uL of Lysozyme solution. Lysozyme activity was measured at 450nm and gave a reading of -0.080 (Abs/min). Calculate the concentration of lysozyme activity in units of abs/min/mL.
4mL of diluted protein solution was prepared by mixing 200uL of the protein solution with 3.8mL of distilled water. 100uL of this diluted was then mixed with 900uL of Lysozyme solution. Lysozyme activity was measured at 450nm and gave a reading of -0.080 (Abs/min). Calculate the concentration of lysozyme activity in units of abs/min/mL.
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.69P
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4mL of diluted protein solution was prepared by mixing 200uL of the protein solution with 3.8mL of distilled water. 100uL of this diluted was then mixed with 900uL of Lysozyme solution. Lysozyme activity was measured at 450nm and gave a reading of -0.080 (Abs/min).
Calculate the concentration of lysozyme activity in units of abs/min/mL.
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