Answered: Report the equation of the calibration…

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Report the equation of the calibration curve and proof of linearity (R2 value).

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter15: Electrophoresis And Other Separation Methods

Section: Chapter Questions

Problem 5P

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Question

Protein ABC is a heme-containing protein responsible in electron transfer in microalgae. The target protein was extracted using ammonium sulfate fractional precipitation and quantified using Bradford assay. A set of solutions with increasing concentration was prepared using 15.00 mg/mL analogous protein standard [refer to succeeding table]. Each solution was treated with 80 μL of Bradford reagent and diluted to 1.500mL. The absorbance at 595 nm of each solution was obtained and presented below.

Volume of protein std, μL	A₅₉₅
0	0.0002
10	0.0007
25	0.0075
50	0.0165
100	0.0356
250	0.0930
500	0.1950
750	0.3010
1000	0.4175

Report the equation of the calibration curve and proof of linearity (R2
value).

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