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How would changing the enzyme concentration affect Km and Vmax

How would changing the enzyme concentration affect Km and Vmax

Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter32: Gas Chromatography
Section: Chapter Questions
Problem 32.22QAP
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I don’t understand how to do part b of question 5. The other picture is my work of part a. How would changing the enzyme concentration affect Km and Vmax. The answer given to us is Vo= 0.796mM/s. I just don’t understand how to get to that answer.
Shift Slope km X (2 uM -- 0.002 mm) 6v - V. max [S] (mM) V (M/s) 0-009 0.860 0.01 1.307 Slope = 1-367 0.860 2.651198 x/0-3 D.01 0.004 5.302396 x10-3 y=mx+b 5.302396 x10-@M Km Vmax [s] Vmax Km Vo Vmax CS] Vmax 2.451198 1.367 0.01 Vmax 6.49999 V max Kcat = Vmax= 2.0000MM/S km Vmax Vmax Krat [E] m.Vmax km= 2.0000 Krat Km = 2.651198 xr03.2 0.002 -5.30 237e x/0-3 appreoched catalytic perfection has = 1600 30 xo-3
Transcribed Image Text:Shift Slope km X (2 uM -- 0.002 mm) 6v - V. max [S] (mM) V (M/s) 0-009 0.860 0.01 1.307 Slope = 1-367 0.860 2.651198 x/0-3 D.01 0.004 5.302396 x10-3 y=mx+b 5.302396 x10-@M Km Vmax [s] Vmax Km Vo Vmax CS] Vmax 2.451198 1.367 0.01 Vmax 6.49999 V max Kcat = Vmax= 2.0000MM/S km Vmax Vmax Krat [E] m.Vmax km= 2.0000 Krat Km = 2.651198 xr03.2 0.002 -5.30 237e x/0-3 appreoched catalytic perfection has = 1600 30 xo-3
4. For an enzyme that has a Km of 25 mM, Vmax of 50 mM/s and kcat of 250 s1, how long does a single reaction take? Hkct= tine of simgle Singe A. 25 s B. 250 ms C. 50 ms D. 4 ms E. 4s 5. A kinetics experiment was performed on enzyme X (2 µM), and gave the data shown below. [S] (uM) V. (mM/s) 4.00 0.860 10.0 1.307 A. Does enzyme X approach catalytic perfection? Justify your answer. B. What will be the reaction rate (Vo) with 1.5 uM enzyme X and 6.0 uM substrate? 6. Why does an enzyme's activity decrease as the temperature rises above its optimal temperature? Why does activity decrease below the optimal temperature? 7. Which of the following statements is true about a competitive enzyme inhibitor? A. It has no effect on Km It binds to the enzyme:substrate complex and prevents it from reacting further C. It decreases the Vmax D. It causes the enzyme to refold into an inactive conformation В. P Type here to search DI ム× F1 F2 F3 F4 F5 F6 @ 2$ % 近 %23
Transcribed Image Text:4. For an enzyme that has a Km of 25 mM, Vmax of 50 mM/s and kcat of 250 s1, how long does a single reaction take? Hkct= tine of simgle Singe A. 25 s B. 250 ms C. 50 ms D. 4 ms E. 4s 5. A kinetics experiment was performed on enzyme X (2 µM), and gave the data shown below. [S] (uM) V. (mM/s) 4.00 0.860 10.0 1.307 A. Does enzyme X approach catalytic perfection? Justify your answer. B. What will be the reaction rate (Vo) with 1.5 uM enzyme X and 6.0 uM substrate? 6. Why does an enzyme's activity decrease as the temperature rises above its optimal temperature? Why does activity decrease below the optimal temperature? 7. Which of the following statements is true about a competitive enzyme inhibitor? A. It has no effect on Km It binds to the enzyme:substrate complex and prevents it from reacting further C. It decreases the Vmax D. It causes the enzyme to refold into an inactive conformation В. P Type here to search DI ム× F1 F2 F3 F4 F5 F6 @ 2$ % 近 %23
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