5. To figure out the initial concentration of a bacterial culture, you made an 8 fold dilution of the initial bacterial culture 10 times. Calculate the overall dilution factor (in other words, what is the dilution factor a) for the last dilution?). Show your work, including the dilution scheme. b) You then plated 100 µl of each dilution for colony counting. After incubation, for the 7th plate, you counted 217 colonies. Based on the available information, calculate the initial bacterial concentration in CFU/ml. Show all of your work, including formula.

5. To figure out the initial concentration of a bacterial culture, you made an 8 fold dilution of the initial bacterial culture 10 times. Calculate the overall dilution factor (in other words, what is the dilution factor a) for the last dilution?). Show your work, including the dilution scheme. b) You then plated 100 µl of each dilution for colony counting. After incubation, for the 7th plate, you counted 217 colonies. Based on the available information, calculate the initial bacterial concentration in CFU/ml. Show all of your work, including formula.

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter4: The Prokaryotes

Section: Chapter Questions

Problem 5UTM

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