6. Assume that you have an “X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. a) Prepare a standard curve by using the absorbance of known concentrations. b) Calculate the concentration of unknown. Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220
6. Assume that you have an “X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. a) Prepare a standard curve by using the absorbance of known concentrations. b) Calculate the concentration of unknown. Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter16: Fiber Optics And Lasers In Bioinstrumentation
Section: Chapter Questions
Problem 6P
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Transcribed Image Text:6. Assume that you have an “X" solution that you do not know its concentration. But you have other
X solutions with known concentrations and you know X solution absorbs maximum light at 575
nm. To calculate the unknown solution concentration, you have done some spectrophotometric
measurements and obtained data given below.
a) Prepare a standard curve by using the absorbance of known concentrations.
b) Calculate the concentration of unknown.
Table 1: Absorbance of X solution (575 nm)
Concentration (mg/ml)
Absorbance
0.025
0.220
0.050
0.440
0.100
0.760
0.150
1.250
0.200
1,460
unknown
0,640
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