6. The qualitative tests for determination of a-amino acids (ninhydrin test, xanthoproteic test, Millon's test, Foli's test) and identification of peptide bond by the biuret test
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- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.1.A 23 year old student was investigated for the cause of his jaundice and puffy face appearance. The results of his chemistry test is as follows: Analyte Results Reference ranges total protein 9.40 g/dl 6.0 – 8.0 g/dl albumin OD=0.09, []=? 3.7 – 5.3 g/dl direct bilirubin 0.30mg/dl ≤0.25mg/dl Total bilirubin 1.20mg/dl ≤ 1.1 mg/dl a. The technician on duty had the optical density of her albumin as 0.09, mean standard absorbance 0.20 and standard concentration of 3.50 g/dl. Calculate i. The concentration of albumin ii. The concentration of globulin iii. The concentration of indirect bilirubinExplain main difference would you see when conducting the nitrous acid test with aniline vs. amino-cyclohexane? Be specific.
- This test will differentiate between aromatic amino acids with an acitivated ring from other amino acids. a) Nihydrin Test b) Xanthoproteic Test C) solubility test d) polarimeter testWhich of the following amino acid can be particularly detected with test using NaOH and lead acetate as reagents? a.tyrosine b.tryptophan c.cysteine d.valineBased on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish ’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.What is the purpose of adding ether in the isolates during the washing steps? Enumerate other techniques that can be used to isolate and characterize protein from a substance and biological specimen (e.g. milk, blood, CSF) Give the general structure of amino acid. Name each partWhat products are formed when below peptide is treated with chymotrypsin? Phe–Tyr–Gly–Cys–Arg–Ser
- 2. a. What ingredient(s) necessary to white chocolate does this product have? b. Why is this product labeled as “white chocolate style” instead of being simply labeled as “White chocolate”?4. Other reagents used in research labs are listed below. What is the meaning of the acronym and what is its purpose? a. Trypsin b. EDTA C. TEDiscuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
