What is the purpose of adding ether in the isolates during the washing steps? Enumerate other techniques that can be used to isolate and characterize protein from a substance and biological specimen (e.g. milk, blood, CSF) Give the general structure of amino acid. Name each part
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- What is the purpose of adding ether in the isolates during the washing steps?
- Enumerate other techniques that can be used to isolate and characterize protein from a substance and biological specimen (e.g. milk, blood, CSF)
- Give the general structure of amino acid. Name each part
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- Provide examples of amino acids that would yield a positive Azo Dye test and amino acids that would yield a negative Azo Dye test. Show the structures.What is the classical method to check the quality of Nucleic Acid Product? Discuss its significanceIn protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8
- Give the chemical principles involved in a positive Biuret test, Millon’s Test and Sakaguchi Test. What is the chemical basis of the Ninhydrin Test, Hopkins – Cole test and Sulfur reaction test? What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test?What chemical test could be used to differentiate a protein from an amino acid? Explain brieflyIf you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.
- What chemical test could be used to differentiate a protein from an amino acid? Explain.What is qualitative analysis of lipids? How important qualitative analysis of lipids in the field of laboratory medicine? Discuss its significance.Tests for Qualitative Analysis of Proteins and Amino Acids: Ninhydrin Test Biuret Test Xanthoproteic Test Millon's Test Hopkins-Cole Test Sakaguchi Test Lead Acetate Test What amino acids are possibly present in each sample tests? Explain why.
- How to identify the proteins and its function using mass spectrometry?How is the biochemist able to isolate a particular protein from a complex mixture of proteins?You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?