6. Which of the following diagrams illustrate(s) an improper way of spotting a TLC plate? Tell what is wrong in each case. a. Oo b. 00 C. 8 d. --Liquid level
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- In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)Why are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table below ) are suitable for HPLC?What is the difference between Pseudocapacitor and EDLC? Can you please explain what happens in pseudocapacitance and EDLC? Which is better? Can you tell me everything i need to know about them?
- If I was in a rush and only let my TLC plate run 1 cm. Am I going to encounter anyissues? If so, what?Please show complete solution. The distance travelled by an unknown sample in the TLC plate is 9 cm. Given the retention factor to be 0.750, calculate the distance travelled by theThin‑layer chromatography (TLC) can be used as a preliminary identification method for many amphetamine‑type stimulants (ATS). Using a 0.25 mm thick silica gel stationary phase and a 98.5:1.5 methanol:concentrated ammonia mobile phase, a spot was observed to have traveled 3.52 cm when the solvent front traveled 8.81 cm. What is the retardation factor (?F)for the compound that composes this spot?
- Define the following terms used in HPLC: (d) sampling loops (e) bonded-phase packing (f) gel filtrationChemistry What is the HETP of a column that is 200mm with a retention time of 5mins. The (effective) theoretical plate number N is 6000. What is the separation factor, a?2. a) What is a bonded phase in HPLC?b) Give an example of a bonded phase in reversed-phase liquid chromatography and normal phase liquid chromatography.c) Describe when you would choose to use a reversed-phase column or a normal-phase column for an analysis of a mixture.
- 1. Define sparging. What is the significance of this step in an HPLC analysis? 2. What are the two types of pre-column? What is the purpose of each type? 3. What are the two types of packing used in HPLC column? Describe each type. Please answer numbers 1-3. Need asapA TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ BWhen the use of 2D Thin layer Chromatography is better than normal TLC? Please shortly answer at your own words. Answer should be to the point (specific 4-5 lines).